Luminol-dependent chemiluminescence analysis of opsonophagocytic dysfunctions

Mark E. Wilson; Michael A. Trush; Knox Van Dyke; James M. Kyle; Martha D. Mullett; William A. Neal

doi:10.1016/0022-1759(78)90206-5

Luminol-dependent chemiluminescence analysis of opsonophagocytic dysfunctions

Mark E. Wilson, Michael A. Trush, Knox Van Dyke, James M. Kyle, Martha D. Mullett, William A. Neal

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

The use of luminol as a means of amplifying the native chemiluminescence responses of phagocytosing human polymorphonuclear leukocytes was studied. Parameters examined included the effects of luminol, leukocyte and particle (opsonized zymosan) concentration on luminol-dependent chemiluminescence response. The results indicate that all of the parameters studied influence the luminol-dependent chemiluminescence assay system and that, on the basis of experiments designed to assess both opsonic and phagocytosis defects, this system appears to reflect the same or similar metabolic events as does native chemiluminescence. The luminol-dependent chemiluminescence assay represents a significant improvement of the phagocyte chemiluminescence assay in that greater sensitivity is possible, and may therefore have clinical utility as a means of evaluating phagocytic function.

Original language	English (US)
Pages (from-to)	315-326
Number of pages	12
Journal	Journal of Immunological Methods
Volume	23
Issue number	3-4
DOIs	https://doi.org/10.1016/0022-1759(78)90206-5
State	Published - Oct 1978
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(78)90206-5

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@article{aa1936399cea41f797591ad315bcfce9,

title = "Luminol-dependent chemiluminescence analysis of opsonophagocytic dysfunctions",

abstract = "The use of luminol as a means of amplifying the native chemiluminescence responses of phagocytosing human polymorphonuclear leukocytes was studied. Parameters examined included the effects of luminol, leukocyte and particle (opsonized zymosan) concentration on luminol-dependent chemiluminescence response. The results indicate that all of the parameters studied influence the luminol-dependent chemiluminescence assay system and that, on the basis of experiments designed to assess both opsonic and phagocytosis defects, this system appears to reflect the same or similar metabolic events as does native chemiluminescence. The luminol-dependent chemiluminescence assay represents a significant improvement of the phagocyte chemiluminescence assay in that greater sensitivity is possible, and may therefore have clinical utility as a means of evaluating phagocytic function.",

author = "Wilson, {Mark E.} and Trush, {Michael A.} and {Van Dyke}, Knox and Kyle, {James M.} and Mullett, {Martha D.} and Neal, {William A.}",

note = "Funding Information: This research was supported by NIH Training Grant GM 07039-03 and by W.V.U. Institutional Grant 2-210-1615 (97).",

year = "1978",

month = oct,

doi = "10.1016/0022-1759(78)90206-5",

language = "English (US)",

volume = "23",

pages = "315--326",

journal = "Journal of Immunological Methods",

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T1 - Luminol-dependent chemiluminescence analysis of opsonophagocytic dysfunctions

AU - Wilson, Mark E.

AU - Trush, Michael A.

AU - Van Dyke, Knox

AU - Kyle, James M.

AU - Mullett, Martha D.

AU - Neal, William A.

N1 - Funding Information: This research was supported by NIH Training Grant GM 07039-03 and by W.V.U. Institutional Grant 2-210-1615 (97).

PY - 1978/10

Y1 - 1978/10

N2 - The use of luminol as a means of amplifying the native chemiluminescence responses of phagocytosing human polymorphonuclear leukocytes was studied. Parameters examined included the effects of luminol, leukocyte and particle (opsonized zymosan) concentration on luminol-dependent chemiluminescence response. The results indicate that all of the parameters studied influence the luminol-dependent chemiluminescence assay system and that, on the basis of experiments designed to assess both opsonic and phagocytosis defects, this system appears to reflect the same or similar metabolic events as does native chemiluminescence. The luminol-dependent chemiluminescence assay represents a significant improvement of the phagocyte chemiluminescence assay in that greater sensitivity is possible, and may therefore have clinical utility as a means of evaluating phagocytic function.

AB - The use of luminol as a means of amplifying the native chemiluminescence responses of phagocytosing human polymorphonuclear leukocytes was studied. Parameters examined included the effects of luminol, leukocyte and particle (opsonized zymosan) concentration on luminol-dependent chemiluminescence response. The results indicate that all of the parameters studied influence the luminol-dependent chemiluminescence assay system and that, on the basis of experiments designed to assess both opsonic and phagocytosis defects, this system appears to reflect the same or similar metabolic events as does native chemiluminescence. The luminol-dependent chemiluminescence assay represents a significant improvement of the phagocyte chemiluminescence assay in that greater sensitivity is possible, and may therefore have clinical utility as a means of evaluating phagocytic function.

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JO - Journal of Immunological Methods

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IS - 3-4

ER -

Luminol-dependent chemiluminescence analysis of opsonophagocytic dysfunctions

Abstract

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