The use of luminol as a means of amplifying the native chemiluminescence responses of phagocytosing human polymorphonuclear leukocytes was studied. Parameters examined included the effects of luminol, leukocyte and particle (opsonized zymosan) concentration on luminol-dependent chemiluminescence response. The results indicate that all of the parameters studied influence the luminol-dependent chemiluminescence assay system and that, on the basis of experiments designed to assess both opsonic and phagocytosis defects, this system appears to reflect the same or similar metabolic events as does native chemiluminescence. The luminol-dependent chemiluminescence assay represents a significant improvement of the phagocyte chemiluminescence assay in that greater sensitivity is possible, and may therefore have clinical utility as a means of evaluating phagocytic function.
ASJC Scopus subject areas
- Immunology and Allergy