Luciferase activity as a marker of tumor burden and as an indicator of tumor response to antineoplastic therapy in vivo

Lurong Zhang, Karl Erik Hellström, Lieping Chen

Research output: Contribution to journalArticle

Abstract

The gene encoding firefly luciferase has been used as a reporter gene for the study of gene function. The luciferase catalyzes its substrate and subsequently produces luminescence. In addition, it is not present in mammalian cells. We have therefore explored its use in monitoring the growth of tumors in vivo. The luciferase gene was transfected into two murine tumor lines, i.e. cl62 melanoma and M109 lung carcinoma, and the luciferase activity associated with the cells was determined by a rapid chemiluminescent reaction. Luciferase activity was well-correlated with the number of tumor cells in vitro. Luciferase activity also correlated with the tumor burden in vivo, as well as with the effect of an adoptive T cell transfer therapy in the syngeneic C3H/HeN mice experimental tumor model. This assay offers the advantage of being quantitative, rapid, and reliable for the detection of tumor burden and for the evaluation of the effect of antineoplastic therapy.

Original languageEnglish (US)
Pages (from-to)87-92
Number of pages6
JournalClinical and Experimental Metastasis
Volume12
Issue number2
DOIs
StatePublished - Mar 1994
Externally publishedYes

Keywords

  • immunotherapy
  • luciferase activity
  • tumor metastasis

ASJC Scopus subject areas

  • Cancer Research

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