Luciferase activity as a marker of tumor burden and as an indicator of tumor response to antineoplastic therapy in vivo

Lurong Zhang, Karl Erik Hellström, Lieping Chen

Research output: Contribution to journalArticle

Abstract

The gene encoding firefly luciferase has been used as a reporter gene for the study of gene function. The luciferase catalyzes its substrate and subsequently produces luminescence. In addition, it is not present in mammalian cells. We have therefore explored its use in monitoring the growth of tumors in vivo. The luciferase gene was transfected into two murine tumor lines, i.e. cl62 melanoma and M109 lung carcinoma, and the luciferase activity associated with the cells was determined by a rapid chemiluminescent reaction. Luciferase activity was well-correlated with the number of tumor cells in vitro. Luciferase activity also correlated with the tumor burden in vivo, as well as with the effect of an adoptive T cell transfer therapy in the syngeneic C3H/HeN mice experimental tumor model. This assay offers the advantage of being quantitative, rapid, and reliable for the detection of tumor burden and for the evaluation of the effect of antineoplastic therapy.

Original languageEnglish (US)
Pages (from-to)87-92
Number of pages6
JournalClinical and Experimental Metastasis
Volume12
Issue number2
DOIs
StatePublished - Mar 1994
Externally publishedYes

Fingerprint

Tumor Burden
Luciferases
Antineoplastic Agents
Neoplasms
Genes
Firefly Luciferases
Therapeutics
Adoptive Transfer
Inbred C3H Mouse
Cell- and Tissue-Based Therapy
Luminescence
Reporter Genes
Melanoma
Theoretical Models
Cell Count
Carcinoma
T-Lymphocytes
Lung
Growth

Keywords

  • immunotherapy
  • luciferase activity
  • tumor metastasis

ASJC Scopus subject areas

  • Cancer Research

Cite this

Luciferase activity as a marker of tumor burden and as an indicator of tumor response to antineoplastic therapy in vivo. / Zhang, Lurong; Hellström, Karl Erik; Chen, Lieping.

In: Clinical and Experimental Metastasis, Vol. 12, No. 2, 03.1994, p. 87-92.

Research output: Contribution to journalArticle

@article{ac42ac521985445e89bdeb4d74c1b9a6,
title = "Luciferase activity as a marker of tumor burden and as an indicator of tumor response to antineoplastic therapy in vivo",
abstract = "The gene encoding firefly luciferase has been used as a reporter gene for the study of gene function. The luciferase catalyzes its substrate and subsequently produces luminescence. In addition, it is not present in mammalian cells. We have therefore explored its use in monitoring the growth of tumors in vivo. The luciferase gene was transfected into two murine tumor lines, i.e. cl62 melanoma and M109 lung carcinoma, and the luciferase activity associated with the cells was determined by a rapid chemiluminescent reaction. Luciferase activity was well-correlated with the number of tumor cells in vitro. Luciferase activity also correlated with the tumor burden in vivo, as well as with the effect of an adoptive T cell transfer therapy in the syngeneic C3H/HeN mice experimental tumor model. This assay offers the advantage of being quantitative, rapid, and reliable for the detection of tumor burden and for the evaluation of the effect of antineoplastic therapy.",
keywords = "immunotherapy, luciferase activity, tumor metastasis",
author = "Lurong Zhang and Hellstr{\"o}m, {Karl Erik} and Lieping Chen",
year = "1994",
month = "3",
doi = "10.1007/BF01753974",
language = "English (US)",
volume = "12",
pages = "87--92",
journal = "Clinical and Experimental Metastasis",
issn = "0262-0898",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Luciferase activity as a marker of tumor burden and as an indicator of tumor response to antineoplastic therapy in vivo

AU - Zhang, Lurong

AU - Hellström, Karl Erik

AU - Chen, Lieping

PY - 1994/3

Y1 - 1994/3

N2 - The gene encoding firefly luciferase has been used as a reporter gene for the study of gene function. The luciferase catalyzes its substrate and subsequently produces luminescence. In addition, it is not present in mammalian cells. We have therefore explored its use in monitoring the growth of tumors in vivo. The luciferase gene was transfected into two murine tumor lines, i.e. cl62 melanoma and M109 lung carcinoma, and the luciferase activity associated with the cells was determined by a rapid chemiluminescent reaction. Luciferase activity was well-correlated with the number of tumor cells in vitro. Luciferase activity also correlated with the tumor burden in vivo, as well as with the effect of an adoptive T cell transfer therapy in the syngeneic C3H/HeN mice experimental tumor model. This assay offers the advantage of being quantitative, rapid, and reliable for the detection of tumor burden and for the evaluation of the effect of antineoplastic therapy.

AB - The gene encoding firefly luciferase has been used as a reporter gene for the study of gene function. The luciferase catalyzes its substrate and subsequently produces luminescence. In addition, it is not present in mammalian cells. We have therefore explored its use in monitoring the growth of tumors in vivo. The luciferase gene was transfected into two murine tumor lines, i.e. cl62 melanoma and M109 lung carcinoma, and the luciferase activity associated with the cells was determined by a rapid chemiluminescent reaction. Luciferase activity was well-correlated with the number of tumor cells in vitro. Luciferase activity also correlated with the tumor burden in vivo, as well as with the effect of an adoptive T cell transfer therapy in the syngeneic C3H/HeN mice experimental tumor model. This assay offers the advantage of being quantitative, rapid, and reliable for the detection of tumor burden and for the evaluation of the effect of antineoplastic therapy.

KW - immunotherapy

KW - luciferase activity

KW - tumor metastasis

UR - http://www.scopus.com/inward/record.url?scp=0028258619&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028258619&partnerID=8YFLogxK

U2 - 10.1007/BF01753974

DO - 10.1007/BF01753974

M3 - Article

VL - 12

SP - 87

EP - 92

JO - Clinical and Experimental Metastasis

JF - Clinical and Experimental Metastasis

SN - 0262-0898

IS - 2

ER -