Low vitamin-D levels combined with PKP3-SIGIRR-TMEM16J host variants is associated with tuberculosis and death in HIV-infected and-exposed infants

Amita Gupta, Grace Montepiedra, Akshay Gupte, Bret Zeldow, Jennifer Jubulis, Barbara Detrick, Avy Violari, Shabir Madhi, Raziya Bobat, Mark Cotton, Charles Mitchell, Stephen Spector

Research output: Contribution to journalArticle

Abstract

Background: This study examined the associations of 25-hydroxyvitamin D and specific host genetic variants that affect vitamin D levels or its effects on immune function, with the risk of TB or mortality in children. Methods: A case-cohort sample of 466 South African infants enrolled in P1041 trial (NCT00080119) underwent 25-hydroxyvitamin D testing by chemiluminescent immunoassay. Single nucleotide polymorphisms (SNPs) that alter the effect of vitamin D [e.g. vitamin D receptor (VDR)], vitamin D levels [e.g. vitamin D binding protein (VDBP)], or toll like receptor (TLR) expression (SIGIRR including adjacent genes PKP3 and TMEM16J) were identified by real-time PCR. Outcomes were time to TB, and to the composite of TB or death by 192 weeks of follow-up. Effect modification between vitamin D status and SNPs for outcomes was assessed. Findings: Median age at 25-hydroxyvitamin D determination was 8 months; 11% were breastfed, 51% were HIV-infected and 26% had low 25-hydroxyvitamin D (

Original languageEnglish (US)
Article numbere0148649
JournalPLoS One
Volume11
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

vitamin D
tuberculosis
Vitamin D
Tuberculosis
HIV
death
Polymorphism
Single Nucleotide Polymorphism
Nucleotides
single nucleotide polymorphism
Vitamin D-Binding Protein
Child Mortality
Calcitriol Receptors
vitamin D-binding protein
Toll-Like Receptors
Immunoassay
Real-Time Polymerase Chain Reaction
immunoassays
Genes
quantitative polymerase chain reaction

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Low vitamin-D levels combined with PKP3-SIGIRR-TMEM16J host variants is associated with tuberculosis and death in HIV-infected and-exposed infants. / Gupta, Amita; Montepiedra, Grace; Gupte, Akshay; Zeldow, Bret; Jubulis, Jennifer; Detrick, Barbara; Violari, Avy; Madhi, Shabir; Bobat, Raziya; Cotton, Mark; Mitchell, Charles; Spector, Stephen.

In: PLoS One, Vol. 11, No. 2, e0148649, 01.02.2016.

Research output: Contribution to journalArticle

Gupta, A, Montepiedra, G, Gupte, A, Zeldow, B, Jubulis, J, Detrick, B, Violari, A, Madhi, S, Bobat, R, Cotton, M, Mitchell, C & Spector, S 2016, 'Low vitamin-D levels combined with PKP3-SIGIRR-TMEM16J host variants is associated with tuberculosis and death in HIV-infected and-exposed infants', PLoS One, vol. 11, no. 2, e0148649. https://doi.org/10.1371/journal.pone.0148649
Gupta, Amita ; Montepiedra, Grace ; Gupte, Akshay ; Zeldow, Bret ; Jubulis, Jennifer ; Detrick, Barbara ; Violari, Avy ; Madhi, Shabir ; Bobat, Raziya ; Cotton, Mark ; Mitchell, Charles ; Spector, Stephen. / Low vitamin-D levels combined with PKP3-SIGIRR-TMEM16J host variants is associated with tuberculosis and death in HIV-infected and-exposed infants. In: PLoS One. 2016 ; Vol. 11, No. 2.
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AU - Jubulis, Jennifer

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AU - Violari, Avy

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