Low molecular weight group IIA and group V phospholipase A2 enzymes have different intracellular locations in mouse bone marrow-derived mast cells

Clifton Bingham, Remond J A Fijneman, Daniel S. Friend, Richard P. Goddeau, Rick A. Rogers, K. Frank Austen, Jonathan P. Arm

Research output: Contribution to journalArticle

Abstract

The subcellular location of the enzymes of eicosanoid biosynthesis is critical for their co-ordinate action in the generation of leukotrienes and prostaglandins. This activity is thought to occur predominantly at a perinuclear location. Whereas the subcellular locations of cytosolic phospholipase (PL) A2 and each of the pathway enzymes of eicosanoid generation have been defined, the distribution of the low molecular weight species of PLA2 has remained elusive because of the lack of antibodies that distinguish among homologous family members. We have prepared affinity- purified rabbit antipeptide IgG antibodies that distinguish mouse group IIA PLA2 and group V PLA2. Immunofluorescence staining and immunogold electron microscopy reveal different subcellular locations for the enzymes. Group IIA2 PLA2 is present in the secretory granules of mouse bone marrow-derived mast cells, consistent with its putative role in facilitating secretory granule exocytosis and its consequent extracellular action. In contrast, group V PLA2 is associated with various membranous organelles including the Golgi apparatus, nuclear envelope, and plasma membrane. The perinuclear location of group V PLA2 is consistent with a putative interaction with translocated cytosolic PLA2 in supplying arachidonic acid for generation of eicosanoid products, while the location in Golgi cisternae may also reflect its action as a secreted enzyme. The spatial segregation of group IIA PLA2 and group V PLA2 implies that these enzymes are not functionally redundant.

Original languageEnglish (US)
Pages (from-to)31476-31484
Number of pages9
JournalJournal of Biological Chemistry
Volume274
Issue number44
DOIs
StatePublished - Oct 29 1999
Externally publishedYes

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Group V Phospholipases A2
Mast Cells
Bone
Molecular Weight
Bone Marrow
Molecular weight
Eicosanoids
Enzymes
Nuclear Envelope
Secretory Vesicles
Cytosolic Phospholipases A2
Antibodies
Leukotrienes
Exocytosis
Golgi Apparatus
Arachidonic Acid
Organelles
Biosynthesis
Prostaglandins
Fluorescent Antibody Technique

ASJC Scopus subject areas

  • Biochemistry

Cite this

Low molecular weight group IIA and group V phospholipase A2 enzymes have different intracellular locations in mouse bone marrow-derived mast cells. / Bingham, Clifton; Fijneman, Remond J A; Friend, Daniel S.; Goddeau, Richard P.; Rogers, Rick A.; Austen, K. Frank; Arm, Jonathan P.

In: Journal of Biological Chemistry, Vol. 274, No. 44, 29.10.1999, p. 31476-31484.

Research output: Contribution to journalArticle

Bingham, Clifton ; Fijneman, Remond J A ; Friend, Daniel S. ; Goddeau, Richard P. ; Rogers, Rick A. ; Austen, K. Frank ; Arm, Jonathan P. / Low molecular weight group IIA and group V phospholipase A2 enzymes have different intracellular locations in mouse bone marrow-derived mast cells. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 44. pp. 31476-31484.
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