TY - JOUR
T1 - Low intensity shear stress increases endothelial ELR+ CXC chemokine production via a focal adhesion kinase-p38β MAPK-NF-κB pathway
AU - Shaik, Sadiq S.
AU - Soltau, Thomas D.
AU - Chaturvedi, Gaurav
AU - Totapally, Balagangadhar
AU - Hagood, James S.
AU - Andrews, William W.
AU - Athar, Mohammad
AU - Voitenok, Nikolai N.
AU - Killingsworth, Cheryl R.
AU - Patel, Rakesh P.
AU - Fallon, Michael B.
AU - Maheshwari, Akhil
PY - 2009/2/27
Y1 - 2009/2/27
N2 - CXC chemokines with a glutamate-leucine-arginine (ELR) tripeptide motif (ELR+ CXC chemokines) play an important role in leukocyte trafficking into the tissues. For reasons that are not well elucidated, circulating leukocytes are recruited into the tissues mainly in small vessels such as capillaries and venules. Because ELR+ CXC chemokines are important mediators of endothelial-leukocyte interaction, we compared chemokine expression by microvascular and aortic endothelium to investigate whether differences in chemokine expression by various endothelial types could, at least partially, explain the microvascular localization of endothelial-leukocyte interaction. Both in vitro and in vivo models indicate that ELR+ CXC chemokine expression is higher in microvascular endothelium than in aortic endothelial cells. These differences can be explained on the basis of the preferential activation of endothelial chemokine production by low intensity shear stress. Low shear activated endothelial ELR+ CXC chemokine production via cell surface heparan sulfates, β 3-integrins, focal adhesion kinase, the mitogen-activated protein kinase p38β, mitogen- and stress-associated protein kinase-1, and the transcription factor.
AB - CXC chemokines with a glutamate-leucine-arginine (ELR) tripeptide motif (ELR+ CXC chemokines) play an important role in leukocyte trafficking into the tissues. For reasons that are not well elucidated, circulating leukocytes are recruited into the tissues mainly in small vessels such as capillaries and venules. Because ELR+ CXC chemokines are important mediators of endothelial-leukocyte interaction, we compared chemokine expression by microvascular and aortic endothelium to investigate whether differences in chemokine expression by various endothelial types could, at least partially, explain the microvascular localization of endothelial-leukocyte interaction. Both in vitro and in vivo models indicate that ELR+ CXC chemokine expression is higher in microvascular endothelium than in aortic endothelial cells. These differences can be explained on the basis of the preferential activation of endothelial chemokine production by low intensity shear stress. Low shear activated endothelial ELR+ CXC chemokine production via cell surface heparan sulfates, β 3-integrins, focal adhesion kinase, the mitogen-activated protein kinase p38β, mitogen- and stress-associated protein kinase-1, and the transcription factor.
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U2 - 10.1074/jbc.M807205200
DO - 10.1074/jbc.M807205200
M3 - Article
C2 - 19117939
AN - SCOPUS:65549117913
SN - 0021-9258
VL - 284
SP - 5945
EP - 5955
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 9
ER -