Low-dose Perampanel rescues cortical gamma dysregulation associated with parvalbumin interneuron GluA2 upregulation in epileptic Syngap1+/- mice

Brennan J. Sullivan, Simon Ammanuel, Pavel A. Kipnis, Yoichi Araki, Richard L. Huganir, Shilpa D. Kadam

Research output: Contribution to journalArticlepeer-review


Loss-of-function SYNGAP1 mutations cause a neurodevelopmental disorder characterized by intellectual disability and epilepsy. SYNGAP1 is a Ras-GTPase-activating protein that underlies the formation and experience-dependent regulation of postsynaptic densities. The mechanisms that contribute to this proposed monogenic cause of intellectual disability and epilepsy remain unresolved. Here, we establish the phenotype of the epileptogenesis in a Syngap1+/- mouse model using 24h video electroencephalogram/electromyogram (vEEG/EMG) recordings at advancing ages. A progressive worsening of clinically-similar seizure phenotypes, interictal spike frequency, sleep dysfunction, and hyperactivity was identified in Syngap1+/- mice. Interictal spikes emerged predominantly during NREM in 24h vEEG of Syngap1+/- mice. Myoclonic seizures occurred at behavioral-state transitions both in Syngap1+/- mice and during an overnight EEG from a child with SYNGAP1 haploinsufficiency. In Syngap1+/- mice, EEG spectral power analyses identified a significant loss of cortical gamma homeostasis during behavioral-state transitions from NREM to Wake and NREM to REM. The loss of gamma homeostasis was associated with a region- and location-specific significant increase of GluA2 AMPA receptor subunit expression in the somas of parvalbumin-positive (PV+) interneurons. Acute dosing with Perampanel, an FDA approved AMPA antagonist significantly rescued cortical gamma homeostasis, identifying a novel mechanism implicating Ca2+ impermeable AMPARs on PV+ interneurons underlying circuit dysfunction in SYNGAP1 haploinsufficiency.

Original languageEnglish (US)
JournalUnknown Journal
StatePublished - Jul 30 2019


  • AMPA receptors
  • Gamma oscillations
  • Interictal spikes
  • Myoclonic seizures
  • NREM
  • Parvalbumin interneurons
  • Perampanel
  • REM
  • Sleep cycles

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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