Low-dose ouabain protects against excitotoxic apoptosis and up-regulates nuclear Bcl-2 in vivo

Research output: Contribution to journalArticle

Abstract

Sodium-potassium ATPase (Na+,K+-ATPase) regulates the electrochemical gradient in cells, thereby providing fluid and ionic homeostasis. Additionally, interaction of the Na+,K+ pump with cardiac glycosides can activate intracellular signaling cascades (resulting in cell growth) and up-regulate transcription factors that promote cell survival. We used an in vivo excitotoxicity model to assess if Na +,K+-ATPase plays a role in neuronal apoptosis. After unilateral, intrastriatal injection of the glutamate receptor agonist kainic acid into postnatal day 7 rats, Na+,K+ pump function was increased at 12 h after excitotoxic challenge, and levels of neuron-specific enzyme subunits were preserved (up to 24 h after injection) in membrane-enriched striatal fractions. In addition, co-injection of kainic acid with a low-dose (0.01nmol) of the cardiac glycoside ouabain significantly (P+,K+-ATPase activity. To evaluate the possible mechanisms for this neuroprotection, we examined the levels of nuclear factor kappa B and Bcl-2 after cardiac glycoside exposure. Low-dose ouabain increased nuclear Bcl-2 (but not nuclear factor kappa B) protein levels at 6 h post injection. Our results suggest that Na+,K+-ATPase allows for progression of apoptosis in excitotoxically-injured neurons, and that sublethal concentrations of ouabain provide neuroprotection against excitotoxicity. The mechanism for this ouabain neuroprotection could be intracellular cascades linked to the Na+,K+-ATPase-ouabain interaction that modulate subcellular Bcl-2 levels. Targeted, therapeutic inhibition of apoptosis through cardiac glycosides may represent an effective strategy against excitotoxicity-mediated neuronal injury.

Original languageEnglish (US)
Pages (from-to)133-144
Number of pages12
JournalNeuroscience
Volume137
Issue number1
DOIs
StatePublished - 2006

Fingerprint

Ouabain
Cardiac Glycosides
Up-Regulation
Apoptosis
Injections
NF-kappa B
Kainic Acid
Excitatory Amino Acid Agonists
Neurons
Corpus Striatum
Sodium-Potassium-Exchanging ATPase
Glutamate Receptors
Adenosine Triphosphatases
Cell Survival
Homeostasis
Transcription Factors
Membranes
sodium-translocating ATPase
Wounds and Injuries
Enzymes

Keywords

  • Brain injury
  • Cell death
  • Glutamate
  • Hypoxic-ischemic encephalopathy
  • Na,K-ATPase
  • Newborn

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Low-dose ouabain protects against excitotoxic apoptosis and up-regulates nuclear Bcl-2 in vivo. / Golden, W Christopher; Martin, Lee J.

In: Neuroscience, Vol. 137, No. 1, 2006, p. 133-144.

Research output: Contribution to journalArticle

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