Low-dose mercury heightens early innate response to coxsackievirus infection in female mice

Kayla L. Penta, De Lisa Fairweather, Devon L. Shirley, Noel R. Rose, Ellen K. Silbergeld, Jennifer F. Nyland

Research output: Contribution to journalArticle

Abstract

Results: As for male mice, mercury exposure significantly increased autoimmune myocarditis and immune infiltration into the heart. During the innate response 6 h post-infection, mercury increased expression of co-stimulatory molecules and innate immune receptors on peritoneal macrophages. At the same time point, the alternatively activated macrophage gene, arginase, was increased while the classically activated macrophage gene, inducible nitric oxide synthase, was unaffected. Expression of activation markers were decreased on peritoneal B cells with mercury exposure while T cells were unaffected. Mercury increased production of pro-inflammatory mediators in the spleen. Macrophage-recruiting chemokines and activating cytokines, such as CCL2, CCL4, and IL-6, were increased with mercury following CVB3 infection.

Conclusions: Thus, mercury treatment exacerbates autoimmune myocarditis in female mice and alters early innate signaling on peritoneal macrophages. Mercury also modulates the cytokine profile in the spleen toward a macrophage-activating milieu, and upregulates alternatively activated macrophage genes, providing evidence that mercury exposure promotes inflammation in the context of infection.

Objective: Mercury is a ubiquitous environmental contaminant with toxic outcomes over a range of exposures. In this study, we investigated the effects of mercury exposure on early immune responses to coxsackievirus B3 (CVB3) infection in a murine model of autoimmune heart disease.

Materials and methods: Female BALB/c mice, susceptible to CVB3-induced autoimmune myocarditis, were treated with mercuric chloride (200 μg/kg body weight every other day for 2 weeks) prior to infection with CVB3. Six hours post-infection, immune cells were isolated from the spleen and peritoneum for flow cytometry, gene expression, and cytokine profiling. Thirty-five days post-infection, hearts were collected for histological examination of immune cell infiltration.

Original languageEnglish (US)
Pages (from-to)31-40
Number of pages10
JournalInflammation Research
Volume64
Issue number1
DOIs
StatePublished - Jan 1 2014

Keywords

  • Autoimmunity
  • Coxsackievirus
  • Cytokine
  • Innate immunity
  • Mercury

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Fingerprint Dive into the research topics of 'Low-dose mercury heightens early innate response to coxsackievirus infection in female mice'. Together they form a unique fingerprint.

  • Cite this