We examined the effect of low density lipoprotein (LDL) on histamine release from purified human lung mast cells. LDL inhibited anti-IgE- induced histamine release in a dose-dependent manner, with 100μg/ml LDL-protein inhibiting histamine release by 83±8% (mean±SEM); half-maximal inhibition occurred at 40-80 μg/ml. LDL also inhibited calcium ionophore A23187-induced histamine release in a dose-dependent manner, with 1 mg/ml of LDL inhibiting histamine release by 83 ± 9%; half maximal inhibition occurred at 220-280 μ/ml. Inhibition by LDL was time-dependent: half-maximal inhibition of anti-IgE- induced histamine release by LDL occurred at 30-50 minutes of incubation. The inhibitory effect of LDL was independent of buffer calcium concentrations (0-5 mM) or temperature (0-37°C). These data are consistent with a newly defined immunoregulatory role for LDL.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Oct 29 1987|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology