Low density lipoprotein (LDL) inhibits histamine release from human mast cells

Edward S. Schulman, Thomas J. Quinn, Thomas J. Post, Phillip O'Donnell, Annabelle Rodriguez, Boas Gonen

Research output: Contribution to journalArticle

Abstract

We examined the effect of low density lipoprotein (LDL) on histamine release from purified human lung mast cells. LDL inhibited anti-IgE- induced histamine release in a dose-dependent manner, with 100μg/ml LDL-protein inhibiting histamine release by 83±8% (mean±SEM); half-maximal inhibition occurred at 40-80 μg/ml. LDL also inhibited calcium ionophore A23187-induced histamine release in a dose-dependent manner, with 1 mg/ml of LDL inhibiting histamine release by 83 ± 9%; half maximal inhibition occurred at 220-280 μ/ml. Inhibition by LDL was time-dependent: half-maximal inhibition of anti-IgE- induced histamine release by LDL occurred at 30-50 minutes of incubation. The inhibitory effect of LDL was independent of buffer calcium concentrations (0-5 mM) or temperature (0-37°C). These data are consistent with a newly defined immunoregulatory role for LDL.

Original languageEnglish (US)
Pages (from-to)553-559
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume148
Issue number2
DOIs
StatePublished - Oct 29 1987
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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