Loss of p53 function in colon cancer cells results in increased phosphocholine and total choline

Noriko Mori, Robert Delsite, Kshama Natarajan, Mariola Kulawiec, Zaver M Bhujwalla, Keshav K. Singh

Research output: Contribution to journalArticle

Abstract

Mutations in the p53 gene are the most frequently observed genetic lesions in human cancers. Human cancers that contain a p53 mutation are more aggressive, more apt to metastasize, and more often fatal. p53 controls numerous downstream targets that can influence various outcomes such as apoptosis, growth arrest, and DNA repair. Based on previous observations using 1H magnetic resonance spectroscopy (MRS), we have Identified choline phospholipid metabolite intensities typical of increased malignancy. Here we have used 1H MRS to characterize the choline phospholipid metabolite levels of p53 +/+ and p53 -/- cells, and demonstrated that loss of p53 function results in Increased phosphocholine and total choline. These data suggest that the Increased malignancy of cancer cells resulting from loss of p53 may be mediated, in part, through the choline phospholipid pathway.

Original languageEnglish (US)
Pages (from-to)319-323
Number of pages5
JournalMolecular Imaging
Volume3
Issue number4
DOIs
Publication statusPublished - Oct 2004

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Keywords

  • Choline phospholipid metabolism
  • Colon cancer cells
  • NMR spectroscopy
  • P53
  • Phosphocholine

ASJC Scopus subject areas

  • Engineering(all)

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