Loss of imprinting of insulin-like growth factor-II (IGF2) gene in distinguishing specific biologic subtypes of Wilms tumor

Jason D. Ravenel, Karl W. Broman, Elizabeth J. Perlman, Emily L. Niemitz, Tilanthi M. Jayawardena, Daphne W. Bell, Daniel A. Haber, Hiroshi Uejima, Andrew P Feinberg

Research output: Contribution to journalArticle

Abstract

Background: Loss of imprinting (LOI) of the insulin-like growth factor-II (IGF2) gene, an epigenetic alteration associated with expression of the normally silent maternal allele, was observed first in Wilms tumor. Although LOI has subsequently been detected in most adult tumors, the biologic role of LOI in cancer remains obscure. We analyzed the imprinting status of Wilms tumors with respect to pathologic subtype, stage, and patient's age at diagnosis and examined the expression of genes potentially affected by LOI. Methods: Of 60 Wilms tumors examined, 25 were informative for an ApaI polymorphism in the IGF2 gene, allowing analysis of allele-specific gene expression, and could be classified by pathologic subtype. Gene expression was measured quantitatively by real-time polymerase chain reaction, and pathologic analysis was blinded for genetic status. All statistical tests were two-sided. Results: We observed LOI of IGF2 in nine (90%) of 10 Wilms tumors classified as having a pathologic subtype associated with a later stage of renal development and in only one (6.7%) of 15 Wilms tumors with a pathologic subtype associated with an earlier stage of renal development (P

Original languageEnglish (US)
Pages (from-to)1698-1703
Number of pages6
JournalJournal of the National Cancer Institute
Volume93
Issue number22
StatePublished - Nov 21 2001

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Insulin-Like Growth Factor II
Wilms Tumor
Genes
Gene Expression
Alleles
Kidney
Epigenomics
Real-Time Polymerase Chain Reaction
Neoplasms
Mothers

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ravenel, J. D., Broman, K. W., Perlman, E. J., Niemitz, E. L., Jayawardena, T. M., Bell, D. W., ... Feinberg, A. P. (2001). Loss of imprinting of insulin-like growth factor-II (IGF2) gene in distinguishing specific biologic subtypes of Wilms tumor. Journal of the National Cancer Institute, 93(22), 1698-1703.

Loss of imprinting of insulin-like growth factor-II (IGF2) gene in distinguishing specific biologic subtypes of Wilms tumor. / Ravenel, Jason D.; Broman, Karl W.; Perlman, Elizabeth J.; Niemitz, Emily L.; Jayawardena, Tilanthi M.; Bell, Daphne W.; Haber, Daniel A.; Uejima, Hiroshi; Feinberg, Andrew P.

In: Journal of the National Cancer Institute, Vol. 93, No. 22, 21.11.2001, p. 1698-1703.

Research output: Contribution to journalArticle

Ravenel, JD, Broman, KW, Perlman, EJ, Niemitz, EL, Jayawardena, TM, Bell, DW, Haber, DA, Uejima, H & Feinberg, AP 2001, 'Loss of imprinting of insulin-like growth factor-II (IGF2) gene in distinguishing specific biologic subtypes of Wilms tumor', Journal of the National Cancer Institute, vol. 93, no. 22, pp. 1698-1703.
Ravenel JD, Broman KW, Perlman EJ, Niemitz EL, Jayawardena TM, Bell DW et al. Loss of imprinting of insulin-like growth factor-II (IGF2) gene in distinguishing specific biologic subtypes of Wilms tumor. Journal of the National Cancer Institute. 2001 Nov 21;93(22):1698-1703.
Ravenel, Jason D. ; Broman, Karl W. ; Perlman, Elizabeth J. ; Niemitz, Emily L. ; Jayawardena, Tilanthi M. ; Bell, Daphne W. ; Haber, Daniel A. ; Uejima, Hiroshi ; Feinberg, Andrew P. / Loss of imprinting of insulin-like growth factor-II (IGF2) gene in distinguishing specific biologic subtypes of Wilms tumor. In: Journal of the National Cancer Institute. 2001 ; Vol. 93, No. 22. pp. 1698-1703.
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abstract = "Background: Loss of imprinting (LOI) of the insulin-like growth factor-II (IGF2) gene, an epigenetic alteration associated with expression of the normally silent maternal allele, was observed first in Wilms tumor. Although LOI has subsequently been detected in most adult tumors, the biologic role of LOI in cancer remains obscure. We analyzed the imprinting status of Wilms tumors with respect to pathologic subtype, stage, and patient's age at diagnosis and examined the expression of genes potentially affected by LOI. Methods: Of 60 Wilms tumors examined, 25 were informative for an ApaI polymorphism in the IGF2 gene, allowing analysis of allele-specific gene expression, and could be classified by pathologic subtype. Gene expression was measured quantitatively by real-time polymerase chain reaction, and pathologic analysis was blinded for genetic status. All statistical tests were two-sided. Results: We observed LOI of IGF2 in nine (90{\%}) of 10 Wilms tumors classified as having a pathologic subtype associated with a later stage of renal development and in only one (6.7{\%}) of 15 Wilms tumors with a pathologic subtype associated with an earlier stage of renal development (P",
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AU - Jayawardena, Tilanthi M.

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AU - Feinberg, Andrew P

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