Abstract
This study examined the frequency of loss of imprinting (LOI) and expression of the insulin-like growth factor 2 (IGF2) gene, and their relationship to selected clinical and pathological factors, in a well defined series of 90 Chinese patients with gastric cancer (GC) and 90 matched patients (controls) diagnosed with nonmalignant conditions. Using peripheral blood and gastric tissue samples, polymerase chain reaction-based assays and restriction endonuclease (Apa I) digestion revealed 33 GC patients and 21 controls to be Apa I informative. LOI of IGF2 was positive in 48.5% (16/33) of primary GC tumor tissues, in 21.2% (7/33) of histologically normal adjacent gastric mucosa (AM) and in 12.1% (4/33) of distant gastric mucosa (DM), and in 15.2% (5/33) of peripheral blood lymphocytes (PBLs). The prevalence of IGF2 LOI in PBL was not statistically different between GC patients (5/33, 15.2%) and control subjects (2/21, 9.5%), P=0.69. Although patients who were found to have LOI of IGF2 were more likely to have advanced stage gastric tumors (P=0.04), no statistically significant differences in survival were found based on imprinting status. IGF2 LOI was associated with an increased expression of IGF2 level in both tumors (P
Original language | English (US) |
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Pages (from-to) | 390-396 |
Number of pages | 7 |
Journal | Molecular Carcinogenesis |
Volume | 50 |
Issue number | 5 |
DOIs | |
State | Published - May 2011 |
Externally published | Yes |
Keywords
- Gastric cancer
- Insulin-like growth factor II
- Loss of imprinting
ASJC Scopus subject areas
- Cancer Research
- Molecular Biology