Loss of heterozygosity on the short arm of chromosome 3 in mesothelioma cell lines and solid tumors

Martha A. Zeiger, James R. Gnarra, Berton Zbar, W. Marston Linehan, Harvey I. Pass

Research output: Contribution to journalArticlepeer-review

Abstract

Cytogenetic analysis of mesothelioma cell lines and solid tumors has documented non‐random chromosomal abnormalities on the short arm of chromosome 3 from 3p 14 to 3p25. We therefore examined nine mesothelioma cell lines, their corresponding tumors, and 15 additional mesothelioma tumors for loss of heterozygosity on 3p from 3p 13 to 3p25.5 by polymerase chain reaction and restriction fragment length polymorphism analysis at 8 loci: D3S3, D3S30, D3S6, D3S2, D3S32, D3FI5S2, THRB, and VHL Loss of heterozygosity was documented by loss of one of two alleles in the tumor DNA whose corresponding normal DNA was heterozygous and was documented in four of nine mesothelioma cell lines and six of 15 mesothelioma tumors or a total of 42% of the mesotheliomas evaluated. This study suggests the involvement of a gene on the short arm of chromosome 3 in the development of mesotheliomas.

Original languageEnglish (US)
Pages (from-to)15-20
Number of pages6
JournalGenes, Chromosomes and Cancer
Volume11
Issue number1
DOIs
StatePublished - Sep 1994

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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