Loss of heterozygosity for loci on the long arm of chromosome 6 in human malignant melanoma

D. Millikin, E. Meese, B. Vogelstein, C. Witkowski, J. Trent

Research output: Contribution to journalArticle

Abstract

Malignant melanoma has been documented to display recurring abnormalities of chromosome 6, particularly the long arm (6q). Restriction fragment length polymorphism analysis was used as a molecular genetic approach to examine loci on chromosome 6q for loss of constitutional heterozygosity (LOH). Five DNA markers that recognize restriction fragment length polymorphisms along 6q and one polymorphic DNA marker for 6p were used to screen 20 autologous pairs of tumor DNA and normal DNA to determine the tumor and constitutional genotypes of each patient. LOH on chromosome 6q was identified at 21 of 53 informative loci (40%). Five patients with more than one informative locus had allele losses consistent with the loss of the entire long arm (or of an entire copy) of chromosome 6, while four other patients demonstrated terminal deletions of 6q. The chromosomal region bearing the highest frequency of 6q allelic loss (60%) is defined by the marker loci c-MYB and ESR (6q22-23 and 6q24-27). In contrast to the frequency of 6q loss, LOH was observed at loci on four other chromosomes (1, 11, 16, 17) in only 5% of cases. These results have led us to conclude that the loss of sequences from the long arm of chromosome 6 is a nonrandom and possibly biologically relevant event in human malignant melanoma.

Original languageEnglish (US)
Pages (from-to)5449-5453
Number of pages5
JournalCancer Research
Volume51
Issue number20
StatePublished - Oct 15 1991
Externally publishedYes

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Chromosomes, Human, Pair 6
Loss of Heterozygosity
Melanoma
Genetic Markers
Restriction Fragment Length Polymorphisms
Chromosomes
Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 1
DNA
Molecular Biology
Neoplasms
Alleles
Genotype

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Millikin, D., Meese, E., Vogelstein, B., Witkowski, C., & Trent, J. (1991). Loss of heterozygosity for loci on the long arm of chromosome 6 in human malignant melanoma. Cancer Research, 51(20), 5449-5453.

Loss of heterozygosity for loci on the long arm of chromosome 6 in human malignant melanoma. / Millikin, D.; Meese, E.; Vogelstein, B.; Witkowski, C.; Trent, J.

In: Cancer Research, Vol. 51, No. 20, 15.10.1991, p. 5449-5453.

Research output: Contribution to journalArticle

Millikin, D, Meese, E, Vogelstein, B, Witkowski, C & Trent, J 1991, 'Loss of heterozygosity for loci on the long arm of chromosome 6 in human malignant melanoma', Cancer Research, vol. 51, no. 20, pp. 5449-5453.
Millikin D, Meese E, Vogelstein B, Witkowski C, Trent J. Loss of heterozygosity for loci on the long arm of chromosome 6 in human malignant melanoma. Cancer Research. 1991 Oct 15;51(20):5449-5453.
Millikin, D. ; Meese, E. ; Vogelstein, B. ; Witkowski, C. ; Trent, J. / Loss of heterozygosity for loci on the long arm of chromosome 6 in human malignant melanoma. In: Cancer Research. 1991 ; Vol. 51, No. 20. pp. 5449-5453.
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