Loss of Adipose Fatty Acid Oxidation Does Not Potentiate Obesity at Thermoneutrality

Research output: Research - peer-reviewArticle

Abstract

Ambient temperature affects energy intake and expenditure to maintain homeostasis in a continuously fluctuating environment. Here, mice with an adipose-specific defect in fatty acid oxidation (Cpt2A-/-) were subjected to varying temperatures to determine the role of adipose bioenergetics in environmental adaptation and body weight regulation. Microarray analysis of mice acclimatized to thermoneutrality revealed that Cpt2A-/- interscapular brown adipose tissue (BAT) failed to induce the expression of thermogenic genes such as Ucp1 and Pgc1α in response to adrenergic stimulation, and increasing ambient temperature exacerbated these defects. Furthermore, thermoneutral housing induced mtDNA stress in Cpt2A-/- BAT and ultimately resulted in a loss of interscapular BAT. Although the loss of adipose fatty acid oxidation resulted in clear molecular, cellular, and physiologic deficits in BAT, body weight gain and glucose tolerance were similar in control and Cpt2A-/- mice in response to a high-fat diet, even when mice were housed at thermoneutrality.

LanguageEnglish (US)
Pages1308-1316
Number of pages9
JournalCell Reports
Volume14
Issue number6
DOIs
StatePublished - Feb 16 2016

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Brown Adipose Tissue
Fatty Acids
Obesity
Tissue
Oxidation
Temperature
Energy Metabolism
Body Weight
Defects
High Fat Diet
Microarray Analysis
Energy Intake
Mitochondrial DNA
Adrenergic Agents
Weight Gain
Homeostasis
Gene Expression
Glucose
Microarrays
Nutrition

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Loss of Adipose Fatty Acid Oxidation Does Not Potentiate Obesity at Thermoneutrality. / Lee, Jieun; Choi, Joseph; Aja, Susan; Scafidi, Susanna; Wolfgang, Michael J.

In: Cell Reports, Vol. 14, No. 6, 16.02.2016, p. 1308-1316.

Research output: Research - peer-reviewArticle

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