Loss of a p53-associated G1 checkpoint does not decrease cell survival following DNA damage

William J. Slichenmyer, William G Nelson, Robbert J. Slebos, Michael B. Kastan

Research output: Contribution to journalArticle

Abstract

Cell cycle checkpoints regulate progression through the cell cycle. In yeast, loss of the G2 checkpoint by mutation of the rad9 gene results in increased genetic instability as well as increased sensitivity to ionizing radiation. In contrast, comparing clonogenic survival of cells which are isogeneic except for p53 functional status, we find that loss of a G1 checkpoint in mammalian cells is not associated with increased sensitivity to the lethal effects of ionizing radiation or a topoisomerase I inhibitor, camptothecin. These results indicate that increased sensitivity to DNA-damaging agents is not necessarily a defining feature of a mammalian cell cycle checkpoint. Furthermore, in light of a recent link of p53 function to radiation-induced apoptosis in hematopoietic cells, these observations suggest that p53-dependent apoptosis is a cell type-specific phenomenon and thus predict that the biological consequences of loss of p53 function will be cell type specific.

Original languageEnglish (US)
Pages (from-to)4164-4168
Number of pages5
JournalCancer Research
Volume53
Issue number18
StatePublished - Sep 15 1993

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DNA Damage
Cell Survival
Cell Cycle Checkpoints
Ionizing Radiation
Topoisomerase I Inhibitors
Apoptosis
Camptothecin
Cell Cycle
Yeasts
Radiation
Mutation
DNA
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Slichenmyer, W. J., Nelson, W. G., Slebos, R. J., & Kastan, M. B. (1993). Loss of a p53-associated G1 checkpoint does not decrease cell survival following DNA damage. Cancer Research, 53(18), 4164-4168.

Loss of a p53-associated G1 checkpoint does not decrease cell survival following DNA damage. / Slichenmyer, William J.; Nelson, William G; Slebos, Robbert J.; Kastan, Michael B.

In: Cancer Research, Vol. 53, No. 18, 15.09.1993, p. 4164-4168.

Research output: Contribution to journalArticle

Slichenmyer, WJ, Nelson, WG, Slebos, RJ & Kastan, MB 1993, 'Loss of a p53-associated G1 checkpoint does not decrease cell survival following DNA damage', Cancer Research, vol. 53, no. 18, pp. 4164-4168.
Slichenmyer, William J. ; Nelson, William G ; Slebos, Robbert J. ; Kastan, Michael B. / Loss of a p53-associated G1 checkpoint does not decrease cell survival following DNA damage. In: Cancer Research. 1993 ; Vol. 53, No. 18. pp. 4164-4168.
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