Long‐term Depression of Glutamate Currents in Cultured Cerebellar Purkinje Neurons Does Not Require Nitric Oxide Signalling

David J. Linden, John A. Connor

Research output: Contribution to journalArticlepeer-review

Abstract

Cerebellar long‐term depression (LTD) is produced when conjunctive stimulation of parallel fibre (PF) and climbing fibre (CF) inputs to a Purkinje neuron (PN) results in a prolonged decrease in the strength of the PF‐PN synapse. In cultured PNs, LTD may be induced by substituting depolarization of the PN and iontophoretic glutamate pulses for CF and PF stimulation, respectively, allowing an unambiguous analysis of post‐synaptic processes (Linden et al., Neuron, 7, 81–89, 1991). Recent studies have suggested that release of the newly described second messenger, nitric oxide, in the cerebellar molecular layer, is necessary for LTD produced in the slice preparation by PF/CF conjunctive stimulation (Shibuki and Okada, Nature, 349, 326–328, 1991) or PF/depolarization conjunctive stimulation (Crepel and Jaillard, NeuroReport, 1, 133–136, 1990). We report that LTD of glutamate currents produced without synaptic stimulation in cultured PNs is unaffected by reagents that stimulate (sodium nitroprusside) or inhibit (haemoglobin, NG‐nitro‐l‐arginine) nitric oxide signalling.

Original languageEnglish (US)
Pages (from-to)10-15
Number of pages6
JournalEuropean Journal of Neuroscience
Volume4
Issue number1
DOIs
StatePublished - Jan 1992
Externally publishedYes

Keywords

  • N‐nitro‐l‐arginine
  • haemoglobin
  • mouse
  • sodium nitroprusside
  • synaptic plasticity

ASJC Scopus subject areas

  • Neuroscience(all)

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