TY - JOUR
T1 - Long‐term Depression of Glutamate Currents in Cultured Cerebellar Purkinje Neurons Does Not Require Nitric Oxide Signalling
AU - Linden, David J.
AU - Connor, John A.
PY - 1992/1
Y1 - 1992/1
N2 - Cerebellar long‐term depression (LTD) is produced when conjunctive stimulation of parallel fibre (PF) and climbing fibre (CF) inputs to a Purkinje neuron (PN) results in a prolonged decrease in the strength of the PF‐PN synapse. In cultured PNs, LTD may be induced by substituting depolarization of the PN and iontophoretic glutamate pulses for CF and PF stimulation, respectively, allowing an unambiguous analysis of post‐synaptic processes (Linden et al., Neuron, 7, 81–89, 1991). Recent studies have suggested that release of the newly described second messenger, nitric oxide, in the cerebellar molecular layer, is necessary for LTD produced in the slice preparation by PF/CF conjunctive stimulation (Shibuki and Okada, Nature, 349, 326–328, 1991) or PF/depolarization conjunctive stimulation (Crepel and Jaillard, NeuroReport, 1, 133–136, 1990). We report that LTD of glutamate currents produced without synaptic stimulation in cultured PNs is unaffected by reagents that stimulate (sodium nitroprusside) or inhibit (haemoglobin, NG‐nitro‐l‐arginine) nitric oxide signalling.
AB - Cerebellar long‐term depression (LTD) is produced when conjunctive stimulation of parallel fibre (PF) and climbing fibre (CF) inputs to a Purkinje neuron (PN) results in a prolonged decrease in the strength of the PF‐PN synapse. In cultured PNs, LTD may be induced by substituting depolarization of the PN and iontophoretic glutamate pulses for CF and PF stimulation, respectively, allowing an unambiguous analysis of post‐synaptic processes (Linden et al., Neuron, 7, 81–89, 1991). Recent studies have suggested that release of the newly described second messenger, nitric oxide, in the cerebellar molecular layer, is necessary for LTD produced in the slice preparation by PF/CF conjunctive stimulation (Shibuki and Okada, Nature, 349, 326–328, 1991) or PF/depolarization conjunctive stimulation (Crepel and Jaillard, NeuroReport, 1, 133–136, 1990). We report that LTD of glutamate currents produced without synaptic stimulation in cultured PNs is unaffected by reagents that stimulate (sodium nitroprusside) or inhibit (haemoglobin, NG‐nitro‐l‐arginine) nitric oxide signalling.
KW - N‐nitro‐l‐arginine
KW - haemoglobin
KW - mouse
KW - sodium nitroprusside
KW - synaptic plasticity
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U2 - 10.1111/j.1460-9568.1992.tb00104.x
DO - 10.1111/j.1460-9568.1992.tb00104.x
M3 - Article
AN - SCOPUS:0026536822
SN - 0953-816X
VL - 4
SP - 10
EP - 15
JO - European Journal of Neuroscience
JF - European Journal of Neuroscience
IS - 1
ER -