Longitudinal study of insulin resistance and sex hormones over the menstrual cycle: The biocycle study

Edwina H. Yeung, Cuilin Zhang, Sunni L. Mumford, Aijun Ye, Maurizio Trevisan, Liwei Chen, Richard W. Browne, Jean Wactawski-Wende, Enrique F. Schisterman

Research output: Contribution to journalArticlepeer-review


Context: Conflicting findings have been reported regarding the effect of menstrual cycle phase and sex hormones on insulin sensitivity. Objective: The aim was to determine the pattern of insulin resistance over the menstrual cycle and whether variations in sex hormones explain these patterns. Design: The BioCycle study is a longitudinal study that measured hormones at different phases of the menstrual cycle. Participants had up to eight visits per cycle; each visit was timed using fertility monitors to capture sensitive windows of hormonal changes. Setting: The study was conducted in the general community of the University at Buffalo (Buffalo, NY). Participants: A total of 257 healthy, premenopausal women (age, 27 ± 8 yr; body mass index, 24 ± 4 kg/m2) participated in the study. Main Outcome Measures: We measured fasting insulin, glucose, and insulin resistance by the homeostasis model of insulin resistance (HOMA-IR). Results: Significant changes in HOMA-IR were observed over the menstrual cycle; from a midfollicular phase level of 1.35, levels rose to 1.59 during the early luteal phase and decreased to 1.55 in the late-luteal phase. HOMA-IR levels primarily reflected changes in insulin and not glucose. After adjustment for age, race, cycle, and other sex hormones, HOMA-IR was positively associated with estradiol (β = 0.082; P <0.001) and progesterone (β = 0.025; P <0.001), and inversely associated with FSH (adjusted β = -0.040; P <0.001) and SHBG (β = -0.085; P <0.001). LH was not associated with HOMA-IR. Further adjustment for BMI weakened the association with SHBG (β = -0.057; P = 0.06) but did not affect other associations. Conclusion: Insulin exhibited minor menstrual cycle variability. Estradiol and progesterone were positively associated with insulin resistance and should be considered in studies of insulin resistance among premenopausal women.

Original languageEnglish (US)
Pages (from-to)5435-5442
Number of pages8
JournalJournal of Clinical Endocrinology and Metabolism
Issue number12
StatePublished - Dec 2010
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism


Dive into the research topics of 'Longitudinal study of insulin resistance and sex hormones over the menstrual cycle: The biocycle study'. Together they form a unique fingerprint.

Cite this