Longitudinal in vivo monitoring of rodent glioma models through thinned skull using laser speckle contrast imaging

Abhishek Rege, Alan C. Seifert, Dan Schlattman, Yu Ouyang, Khan W. Li, Luca Basaldella, Henry Brem, Betty M. Tyler, Nitish V. Thakor

Research output: Contribution to journalArticle

Abstract

Laser speckle contrast imaging (LSCI) is a contrast agent free imaging technique suited for longitudinal assessment of vascular remodeling that accompanies brain tumor growth. We report the use of LSCI to monitor vascular changes in a rodent glioma model. Ten rats are inoculated with 9L gliosarcoma cells, and the angiogenic response is monitored five times over two weeks through a thinned skull imaging window. We are able to visualize neovascularization and measure the number of vessels per unit area to assess quantitatively the microvessel density (MVD). Spatial spread of MVD reveals regions of high MVD that may correspond to tumor location. Whole-field average MVD values increase with time in the tumor group but are fairly stable in the control groups. Statistical analysis shows significant differences in MVD values between the tumor group and both saline-receiving and unperturbed control groups over the two-week period (p < 0.05 ). In conclusion, LSCI is suitable for investigation of tumor angiogenesis in rodent models. In addition, the statistical difference (p < 0.02) between MVD values of the tumor (24.40 ± 1.41) and control groups ( 15.40 ± 1.60) on the 14th day after inoculation suggests a potential use of LSCI in the clinic in distinguishing tumor environments from normal vasculature.

Original languageEnglish (US)
Article number126017
JournalJournal of biomedical optics
Volume17
Issue number12
DOIs
StatePublished - Dec 2012

Keywords

  • Brain tumor angiogenesis
  • Functional vascular density
  • Laser speckle contrast imaging
  • Microvessel density

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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