Longitudinal analysis of simian immunodeficiency virus (SIV) replication in the lungs: Compartmentalized regulation of SIV

Sheila A. Barber, Lucio Gama, Ming Li, Tauni Voelker, John E. Anderson, M. Christine Zink, Patrick M. Tarwater, Lucy M. Carruth, Janice E. Clements

Research output: Contribution to journalArticlepeer-review


Background. Before the onset of AIDS, replication of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) in the lungs is considered to be latent. When and how virus replication is controlled in the lungs is unclear. In the present study, we examine virus replication in the lungs and in cells recovered from bronchoalveolar lavage (BAL) samples in a comprehensive, longitudinal analysis of an SIV/macaque model. Methods. Gene-specific RNA and DNA were quantitated by polymerase chain reaction (PCR) and by real-time reverse-transcription PCR (RT-PCR). Alveolar macrophages were isolated using Dynabeads CD14 (Invitrogen). Expression of CCAAT/enhancer-binding protein β (C/EBPβ) isoforms was examined by Western blot analysis. Results. SIV replication occurred in the lungs during acute infection and correlated with plasma viral load. Innate immune responses involving interferon-β and the dominant-negative isoform of C/EBPβ were induced at this time. SIV RNA expression was suppressed in the lungs during asymptomatic infection, when no correlation existed with plasma viral load until SIV RNA levels rebounded again during late-stage disease. Modulation of viral RNA levels in BAL cells reflected RNA levels in lung tissue throughout each phase of infection. Conclusion. Quantitation of SIV RNA in BAL cells provides a consistent surrogate assessment of virus replication in lung tissue. Innate immune responses contribute to compartmentalized suppression of acute SIV replication in the lungs.

Original languageEnglish (US)
Pages (from-to)931-938
Number of pages8
JournalJournal of Infectious Diseases
Issue number7
StatePublished - Oct 1 2006

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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