Longitudinal analysis of plasmodium sporozoite motility in the dermis reveals component of blood vessel recognition

Christine S. Hopp, Kevin Chiou, Daniel R.T. Ragheb, Ahmed M. Salman, Shahid M. Khan, Andrea J. Liu, Photini Sinnis

Research output: Contribution to journalArticle

Abstract

Malaria infection starts with injection of Plasmodium sporozoites by an Anopheles mosquito into the skin of the mammalian host. How sporozoites locate and enter a blood vessel is a critical, but poorly understood process. In this study, we examine sporozoite motility and their interaction with dermal blood vessels, using intravital microscopy in mice. Our data suggest that sporozoites exhibit two types of motility: in regions far from blood vessels, they exhibit ‘avascular motility’, defined by high speed and less confinement, while in the vicinity of blood vessels their motility is more constrained. We find that curvature of sporozoite tracks engaging with vasculature optimizes contact with dermal capillaries. Imaging of sporozoites with mutations in key adhesive proteins highlight the importance of the sporozoite’s gliding speed and its ability to modulate adhesive properties for successful exit from the inoculation site.

Original languageEnglish (US)
Article numbere07789
JournaleLife
Volume4
Issue numberAUGUST2015
DOIs
StatePublished - Aug 13 2015

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Longitudinal analysis of plasmodium sporozoite motility in the dermis reveals component of blood vessel recognition'. Together they form a unique fingerprint.

  • Cite this

    Hopp, C. S., Chiou, K., Ragheb, D. R. T., Salman, A. M., Khan, S. M., Liu, A. J., & Sinnis, P. (2015). Longitudinal analysis of plasmodium sporozoite motility in the dermis reveals component of blood vessel recognition. eLife, 4(AUGUST2015), [e07789]. https://doi.org/10.7554/eLife.07789