Long-term molecular and cellular stability of human neural stem cell lines

Ana Villa, Beatriz Navarro-Galve, Carlos Bueno, Sonia Franco, María A. Blasco, Alberto Martinez-Serrano

Research output: Contribution to journalArticle

Abstract

Human Neural Stem Cells (hNSCs) are excellent candidates for in vitro and in vivo molecular, cellular, and developmental research, and also for ex-vivo gene transfer and cell therapy in the nervous system. However, hNSCs are mortal somatic cells, and thus invariably enter an irreversible growth arrest after a finite number of cell divisions in culture. It has been proposed that this is due to telomere shortening. Here, we show that long-term cultured (up to 4 years) v-myc perpetuated hNSC lines do preserve short but stable and homogeneous telomeres (TRF and Q-FISH determinations). hNSC lines (but not strains) express high levels of telomerase activity, which is activated by v-myc, as demonstrated here. Telomerase activity is not constitutive, becoming non-detectable after differentiation (in parallel to v-myc down-regulation). hNSC lines also maintain a stable cell cycle length, mitotic potential, differentiation and neuron generation capacity, and do not express senescence-associated β-galactosidase over years, as studied here. These data, collectively, help to explain the immortal nature of v-myc-perpetuated hNSC lines, and to establish them as excellent research tools for basic and applied neurobiological and translational studies.

Original languageEnglish (US)
Pages (from-to)559-570
Number of pages12
JournalExperimental Cell Research
Volume294
Issue number2
DOIs
StatePublished - Apr 1 2004
Externally publishedYes

Fingerprint

Neural Stem Cells
Cell Line
Telomerase
Galactosidases
Telomere Shortening
Telomere
Cell- and Tissue-Based Therapy
Research
Genetic Therapy
Cell Division
Nervous System
Cell Cycle
Down-Regulation
Neurons
Growth

Keywords

  • hNSCs
  • Human neural stem cells
  • Immortal
  • PD
  • Population doubling
  • Q-FISH
  • Quantitative fluorescent in situ hybridization
  • SA-β-gal
  • Senescence
  • Senescence associated β-galactosidase
  • Telomerase
  • Telomere
  • TRF

ASJC Scopus subject areas

  • Cell Biology

Cite this

Villa, A., Navarro-Galve, B., Bueno, C., Franco, S., Blasco, M. A., & Martinez-Serrano, A. (2004). Long-term molecular and cellular stability of human neural stem cell lines. Experimental Cell Research, 294(2), 559-570. https://doi.org/10.1016/j.yexcr.2003.11.025

Long-term molecular and cellular stability of human neural stem cell lines. / Villa, Ana; Navarro-Galve, Beatriz; Bueno, Carlos; Franco, Sonia; Blasco, María A.; Martinez-Serrano, Alberto.

In: Experimental Cell Research, Vol. 294, No. 2, 01.04.2004, p. 559-570.

Research output: Contribution to journalArticle

Villa, A, Navarro-Galve, B, Bueno, C, Franco, S, Blasco, MA & Martinez-Serrano, A 2004, 'Long-term molecular and cellular stability of human neural stem cell lines', Experimental Cell Research, vol. 294, no. 2, pp. 559-570. https://doi.org/10.1016/j.yexcr.2003.11.025
Villa A, Navarro-Galve B, Bueno C, Franco S, Blasco MA, Martinez-Serrano A. Long-term molecular and cellular stability of human neural stem cell lines. Experimental Cell Research. 2004 Apr 1;294(2):559-570. https://doi.org/10.1016/j.yexcr.2003.11.025
Villa, Ana ; Navarro-Galve, Beatriz ; Bueno, Carlos ; Franco, Sonia ; Blasco, María A. ; Martinez-Serrano, Alberto. / Long-term molecular and cellular stability of human neural stem cell lines. In: Experimental Cell Research. 2004 ; Vol. 294, No. 2. pp. 559-570.
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