Long-term hydrocephalus alters the cytoarchitecture of the adult subventricular zone

Tania Campos-Ordoñez, Vicente Herranz-Pérez, Kaisorn L. Chaichana, Jordina Rincon-Torroella, Daniele Rigamonti, Jose M. García-Verdugo, Alfredo Quiñones-Hinojosa, Oscar Gonzalez-Perez

Research output: Contribution to journalArticle

Abstract

Hydrocephalus can develop secondarily to a disturbance in production, flow and/or absorption of cerebrospinal fluid. Experimental models of hydrocephalus, especially subacute and chronic hydrocephalus, are few and limited, and the effects of hydrocephalus on the subventricular zone are unclear. The aim of this study was to analyze the effects of long-term obstructive hydrocephalus on the subventricular zone, which is the neurogenic niche lining the lateral ventricles. We developed a new method to induce hydrocephalus by obstructing the aqueduct of Sylvius in the mouse brain, thus simulating aqueductal stenosis in humans. In 120-day-old rodents (n. = 18 per group), the degree of ventricular dilatation and cellular composition of the subventricular zone were studied by immunofluorescence and transmission electron microscopy. In adult patients (age. >. 18. years), the sizes of the subventricular zone, corpus callosum, and internal capsule were analyzed by magnetic resonance images obtained from patients with and without aqueductal stenosis (n. = 25 per group). Mice with 60-day hydrocephalus had a reduced number of Ki67. + and doublecortin. +. cells on immunofluorescence, as well as decreased number of neural progenitors and neuroblasts in the subventricular zone on electron microscopy analysis as compared to non-hydrocephalic mice. Remarkably, a number of extracellular matrix structures (fractones) contacting the ventricular lumen and blood vessels were also observed around the subventricular zone in mice with hydrocephalus. In humans, the widths of the subventricular zone, corpus callosum, and internal capsule in patients with aqueductal stenosis were significantly smaller than age and gender-matched patients without aqueductal stenosis. In summary, supratentorial hydrocephalus reduces the proliferation rate of neural progenitors and modifies the cytoarchitecture and extracellular matrix compounds of the subventricular zone. In humans, this similar process reduces the subventricular niche as well as the width of corpus callosum and internal capsule.

Original languageEnglish (US)
Pages (from-to)236-244
Number of pages9
JournalExperimental Neurology
Volume261
DOIs
StatePublished - 2014

Fingerprint

Lateral Ventricles
Hydrocephalus
Internal Capsule
Corpus Callosum
Fluorescent Antibody Technique
Extracellular Matrix
Cerebral Aqueduct
Transmission Electron Microscopy
Blood Vessels
Cerebrospinal Fluid
Dilatation
Rodentia
Electron Microscopy
Theoretical Models
Magnetic Resonance Spectroscopy

Keywords

  • Aqueductal stenosis
  • Fractones
  • Human brain
  • Hydrocephalus
  • Neural stem cells
  • Neurogenesis
  • Subventricular zone

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience
  • Medicine(all)

Cite this

Campos-Ordoñez, T., Herranz-Pérez, V., Chaichana, K. L., Rincon-Torroella, J., Rigamonti, D., García-Verdugo, J. M., ... Gonzalez-Perez, O. (2014). Long-term hydrocephalus alters the cytoarchitecture of the adult subventricular zone. Experimental Neurology, 261, 236-244. https://doi.org/10.1016/j.expneurol.2014.05.011

Long-term hydrocephalus alters the cytoarchitecture of the adult subventricular zone. / Campos-Ordoñez, Tania; Herranz-Pérez, Vicente; Chaichana, Kaisorn L.; Rincon-Torroella, Jordina; Rigamonti, Daniele; García-Verdugo, Jose M.; Quiñones-Hinojosa, Alfredo; Gonzalez-Perez, Oscar.

In: Experimental Neurology, Vol. 261, 2014, p. 236-244.

Research output: Contribution to journalArticle

Campos-Ordoñez, T, Herranz-Pérez, V, Chaichana, KL, Rincon-Torroella, J, Rigamonti, D, García-Verdugo, JM, Quiñones-Hinojosa, A & Gonzalez-Perez, O 2014, 'Long-term hydrocephalus alters the cytoarchitecture of the adult subventricular zone', Experimental Neurology, vol. 261, pp. 236-244. https://doi.org/10.1016/j.expneurol.2014.05.011
Campos-Ordoñez T, Herranz-Pérez V, Chaichana KL, Rincon-Torroella J, Rigamonti D, García-Verdugo JM et al. Long-term hydrocephalus alters the cytoarchitecture of the adult subventricular zone. Experimental Neurology. 2014;261:236-244. https://doi.org/10.1016/j.expneurol.2014.05.011
Campos-Ordoñez, Tania ; Herranz-Pérez, Vicente ; Chaichana, Kaisorn L. ; Rincon-Torroella, Jordina ; Rigamonti, Daniele ; García-Verdugo, Jose M. ; Quiñones-Hinojosa, Alfredo ; Gonzalez-Perez, Oscar. / Long-term hydrocephalus alters the cytoarchitecture of the adult subventricular zone. In: Experimental Neurology. 2014 ; Vol. 261. pp. 236-244.
@article{abaeabe5be0e4ed39e8bf17978fbb7ba,
title = "Long-term hydrocephalus alters the cytoarchitecture of the adult subventricular zone",
abstract = "Hydrocephalus can develop secondarily to a disturbance in production, flow and/or absorption of cerebrospinal fluid. Experimental models of hydrocephalus, especially subacute and chronic hydrocephalus, are few and limited, and the effects of hydrocephalus on the subventricular zone are unclear. The aim of this study was to analyze the effects of long-term obstructive hydrocephalus on the subventricular zone, which is the neurogenic niche lining the lateral ventricles. We developed a new method to induce hydrocephalus by obstructing the aqueduct of Sylvius in the mouse brain, thus simulating aqueductal stenosis in humans. In 120-day-old rodents (n. = 18 per group), the degree of ventricular dilatation and cellular composition of the subventricular zone were studied by immunofluorescence and transmission electron microscopy. In adult patients (age. >. 18. years), the sizes of the subventricular zone, corpus callosum, and internal capsule were analyzed by magnetic resonance images obtained from patients with and without aqueductal stenosis (n. = 25 per group). Mice with 60-day hydrocephalus had a reduced number of Ki67. + and doublecortin. +. cells on immunofluorescence, as well as decreased number of neural progenitors and neuroblasts in the subventricular zone on electron microscopy analysis as compared to non-hydrocephalic mice. Remarkably, a number of extracellular matrix structures (fractones) contacting the ventricular lumen and blood vessels were also observed around the subventricular zone in mice with hydrocephalus. In humans, the widths of the subventricular zone, corpus callosum, and internal capsule in patients with aqueductal stenosis were significantly smaller than age and gender-matched patients without aqueductal stenosis. In summary, supratentorial hydrocephalus reduces the proliferation rate of neural progenitors and modifies the cytoarchitecture and extracellular matrix compounds of the subventricular zone. In humans, this similar process reduces the subventricular niche as well as the width of corpus callosum and internal capsule.",
keywords = "Aqueductal stenosis, Fractones, Human brain, Hydrocephalus, Neural stem cells, Neurogenesis, Subventricular zone",
author = "Tania Campos-Ordo{\~n}ez and Vicente Herranz-P{\'e}rez and Chaichana, {Kaisorn L.} and Jordina Rincon-Torroella and Daniele Rigamonti and Garc{\'i}a-Verdugo, {Jose M.} and Alfredo Qui{\~n}ones-Hinojosa and Oscar Gonzalez-Perez",
year = "2014",
doi = "10.1016/j.expneurol.2014.05.011",
language = "English (US)",
volume = "261",
pages = "236--244",
journal = "Experimental Neurology",
issn = "0014-4886",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Long-term hydrocephalus alters the cytoarchitecture of the adult subventricular zone

AU - Campos-Ordoñez, Tania

AU - Herranz-Pérez, Vicente

AU - Chaichana, Kaisorn L.

AU - Rincon-Torroella, Jordina

AU - Rigamonti, Daniele

AU - García-Verdugo, Jose M.

AU - Quiñones-Hinojosa, Alfredo

AU - Gonzalez-Perez, Oscar

PY - 2014

Y1 - 2014

N2 - Hydrocephalus can develop secondarily to a disturbance in production, flow and/or absorption of cerebrospinal fluid. Experimental models of hydrocephalus, especially subacute and chronic hydrocephalus, are few and limited, and the effects of hydrocephalus on the subventricular zone are unclear. The aim of this study was to analyze the effects of long-term obstructive hydrocephalus on the subventricular zone, which is the neurogenic niche lining the lateral ventricles. We developed a new method to induce hydrocephalus by obstructing the aqueduct of Sylvius in the mouse brain, thus simulating aqueductal stenosis in humans. In 120-day-old rodents (n. = 18 per group), the degree of ventricular dilatation and cellular composition of the subventricular zone were studied by immunofluorescence and transmission electron microscopy. In adult patients (age. >. 18. years), the sizes of the subventricular zone, corpus callosum, and internal capsule were analyzed by magnetic resonance images obtained from patients with and without aqueductal stenosis (n. = 25 per group). Mice with 60-day hydrocephalus had a reduced number of Ki67. + and doublecortin. +. cells on immunofluorescence, as well as decreased number of neural progenitors and neuroblasts in the subventricular zone on electron microscopy analysis as compared to non-hydrocephalic mice. Remarkably, a number of extracellular matrix structures (fractones) contacting the ventricular lumen and blood vessels were also observed around the subventricular zone in mice with hydrocephalus. In humans, the widths of the subventricular zone, corpus callosum, and internal capsule in patients with aqueductal stenosis were significantly smaller than age and gender-matched patients without aqueductal stenosis. In summary, supratentorial hydrocephalus reduces the proliferation rate of neural progenitors and modifies the cytoarchitecture and extracellular matrix compounds of the subventricular zone. In humans, this similar process reduces the subventricular niche as well as the width of corpus callosum and internal capsule.

AB - Hydrocephalus can develop secondarily to a disturbance in production, flow and/or absorption of cerebrospinal fluid. Experimental models of hydrocephalus, especially subacute and chronic hydrocephalus, are few and limited, and the effects of hydrocephalus on the subventricular zone are unclear. The aim of this study was to analyze the effects of long-term obstructive hydrocephalus on the subventricular zone, which is the neurogenic niche lining the lateral ventricles. We developed a new method to induce hydrocephalus by obstructing the aqueduct of Sylvius in the mouse brain, thus simulating aqueductal stenosis in humans. In 120-day-old rodents (n. = 18 per group), the degree of ventricular dilatation and cellular composition of the subventricular zone were studied by immunofluorescence and transmission electron microscopy. In adult patients (age. >. 18. years), the sizes of the subventricular zone, corpus callosum, and internal capsule were analyzed by magnetic resonance images obtained from patients with and without aqueductal stenosis (n. = 25 per group). Mice with 60-day hydrocephalus had a reduced number of Ki67. + and doublecortin. +. cells on immunofluorescence, as well as decreased number of neural progenitors and neuroblasts in the subventricular zone on electron microscopy analysis as compared to non-hydrocephalic mice. Remarkably, a number of extracellular matrix structures (fractones) contacting the ventricular lumen and blood vessels were also observed around the subventricular zone in mice with hydrocephalus. In humans, the widths of the subventricular zone, corpus callosum, and internal capsule in patients with aqueductal stenosis were significantly smaller than age and gender-matched patients without aqueductal stenosis. In summary, supratentorial hydrocephalus reduces the proliferation rate of neural progenitors and modifies the cytoarchitecture and extracellular matrix compounds of the subventricular zone. In humans, this similar process reduces the subventricular niche as well as the width of corpus callosum and internal capsule.

KW - Aqueductal stenosis

KW - Fractones

KW - Human brain

KW - Hydrocephalus

KW - Neural stem cells

KW - Neurogenesis

KW - Subventricular zone

UR - http://www.scopus.com/inward/record.url?scp=84905449577&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84905449577&partnerID=8YFLogxK

U2 - 10.1016/j.expneurol.2014.05.011

DO - 10.1016/j.expneurol.2014.05.011

M3 - Article

VL - 261

SP - 236

EP - 244

JO - Experimental Neurology

JF - Experimental Neurology

SN - 0014-4886

ER -