Long-term culture of human esophageal explants and cells

James H. Resau, Patricia C. Phelps, Si min Zhu, Duane Smoot, Hsiang Kuang Lee, John R. Cottrell, Eric A. Hudson, Kathryn A. Elliget

Research output: Contribution to journalArticle

Abstract

Human esophageal epithelium obtained from intermediate autopsies (3H-TdR tabelling in KGM cultures when compared to control cells grown in KGM without EGF (KBM). The morphologic pattern of the cytoskeletal proteins actin, keratin, and vimentin were characterized in isolated cell populations. The intermediate filaments, keratin, and vimentin were co-expressed in these epithelial cells. Esophageal explant viability, differentiation, and outgrowth from 15 cases were also evaluated in dishes coated with basement membrane associated proteins. Explants cultured in these dishes were equally well-preserved and differentiated. There were no significant differences in the explant histology when there was protein coating of the culture dishes, although one case showed improved outgrowth with laminin coating. A main advantage for using this culture system is that the same medium (KGM) can be used for both the culture of explants and isolated epithelial cells. Future applications of this model include determining: (1) the effect different concentrations of EGF and calcium in the media will have on esophageal proliferation and differentiation, and (2) the role of different basement membrane associated proteins on the plating efficiency of either isolated or outgrowth epithelial esophageal cells.

Original languageEnglish (US)
Pages (from-to)61-73
Number of pages13
JournalCytotechnology
Volume3
Issue number1
DOIs
StatePublished - Jan 1990
Externally publishedYes

Fingerprint

Epithelial Cells
Vimentin
Keratins
Proteins
Keratin
Epidermal Growth Factor
Basement Membrane
Membrane Proteins
Cells
Coatings
Histology
Cytoskeletal Proteins
Intermediate Filaments
Laminin
Cell culture
Plating
Actins
Autopsy
Epithelium
Calcium

Keywords

  • epithelium
  • esophagus
  • organ culture

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Biotechnology
  • Medicine(all)

Cite this

Resau, J. H., Phelps, P. C., Zhu, S. M., Smoot, D., Lee, H. K., Cottrell, J. R., ... Elliget, K. A. (1990). Long-term culture of human esophageal explants and cells. Cytotechnology, 3(1), 61-73. https://doi.org/10.1007/BF00365267

Long-term culture of human esophageal explants and cells. / Resau, James H.; Phelps, Patricia C.; Zhu, Si min; Smoot, Duane; Lee, Hsiang Kuang; Cottrell, John R.; Hudson, Eric A.; Elliget, Kathryn A.

In: Cytotechnology, Vol. 3, No. 1, 01.1990, p. 61-73.

Research output: Contribution to journalArticle

Resau, JH, Phelps, PC, Zhu, SM, Smoot, D, Lee, HK, Cottrell, JR, Hudson, EA & Elliget, KA 1990, 'Long-term culture of human esophageal explants and cells', Cytotechnology, vol. 3, no. 1, pp. 61-73. https://doi.org/10.1007/BF00365267
Resau JH, Phelps PC, Zhu SM, Smoot D, Lee HK, Cottrell JR et al. Long-term culture of human esophageal explants and cells. Cytotechnology. 1990 Jan;3(1):61-73. https://doi.org/10.1007/BF00365267
Resau, James H. ; Phelps, Patricia C. ; Zhu, Si min ; Smoot, Duane ; Lee, Hsiang Kuang ; Cottrell, John R. ; Hudson, Eric A. ; Elliget, Kathryn A. / Long-term culture of human esophageal explants and cells. In: Cytotechnology. 1990 ; Vol. 3, No. 1. pp. 61-73.
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