Long-Term Cigarette Smoke Exposure and Changes in MiRNA Expression and Proteome in Non-Small-Cell Lung Cancer

Jayshree Advani, Yashwanth Subbannayya, Krishna Patel, Aafaque Ahmad Khan, Arun H. Patil, Ankit P. Jain, Hitendra S. Solanki, Aneesha Radhakrishnan, Sneha M. Pinto, Nandini A. Sahasrabuddhe, Joji K. Thomas, Premendu P. Mathur, Bipin G. Nair, Xiaofei Chang, T. S.Keshava Prasad, David Sidransky, Harsha Gowda, Aditi Chatterjee

Research output: Contribution to journalArticle

Abstract

Chronic exposure to cigarette smoke markedly increases the risk for lung cancer. Regulation of gene expression at the post-transcriptional level by miRNAs influences a variety of cancer-related interactomes. Yet, relatively little is known on the effects of long-term cigarette smoke exposure on miRNA expression and gene regulation. NCI-H292 (H292) is a cell line sensitive to cigarette smoke with mucoepidermoid characteristics in culture. We report, in this study, original observations on long-term (12 months) cigarette smoke effects in the H292 cell line, using microarray-based miRNA expression profiling, and stable isotopic labeling with amino acids in cell culture-based quantitative proteomic analysis. We identified 112 upregulated and 147 downregulated miRNAs (by twofold) in cigarette smoke-treated H292 cells. The liquid chromatography-tandem mass spectrometry analysis identified 3,959 proteins, of which, 303 proteins were overexpressed and 112 proteins downregulated (by twofold). We observed 39 miRNA target pairs (proven targets) that were differentially expressed in response to chronic cigarette smoke exposure. Gene ontology analysis of the target proteins revealed enrichment of proteins in biological processes driving metabolism, cell communication, and nucleic acid metabolism. Pathway analysis revealed the enrichment of phagosome maturation, antigen presentation pathway, nuclear factor erythroid 2-related factor 2-mediated oxidative stress response, and cholesterol biosynthesis pathways in cigarette smoke-exposed cells. In conclusion, this report makes an important contribution to knowledge on molecular changes in a lung cell line in response to long term cigarette smoke exposure. The findings might inform future strategies for drug target, biomarker and diagnostics innovation in lung cancer, and clinical oncology. These observations also call for further research on the extent to which continuing or stopping cigarette smoking in patients diagnosed with lung cancer translates into molecular and clinical outcomes.

Original languageEnglish (US)
Pages (from-to)390-403
Number of pages14
JournalOMICS A Journal of Integrative Biology
Volume21
Issue number7
DOIs
StatePublished - Jul 1 2017

Fingerprint

Proteome
Non-Small Cell Lung Carcinoma
Smoke
Tobacco Products
Cells
MicroRNAs
Lung Neoplasms
Gene Expression Regulation
Proteins
Cell Line
Metabolism
Gene expression
Down-Regulation
Biological Phenomena
Phagosomes
Gene Ontology
Medical Oncology
Antigen Presentation
Oxidative stress
Oncology

Keywords

  • biomarkers
  • cigarette smoke
  • diagnostics
  • Lung cancer
  • miRNA profiling

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Advani, J., Subbannayya, Y., Patel, K., Khan, A. A., Patil, A. H., Jain, A. P., ... Chatterjee, A. (2017). Long-Term Cigarette Smoke Exposure and Changes in MiRNA Expression and Proteome in Non-Small-Cell Lung Cancer. OMICS A Journal of Integrative Biology, 21(7), 390-403. https://doi.org/10.1089/omi.2017.0045

Long-Term Cigarette Smoke Exposure and Changes in MiRNA Expression and Proteome in Non-Small-Cell Lung Cancer. / Advani, Jayshree; Subbannayya, Yashwanth; Patel, Krishna; Khan, Aafaque Ahmad; Patil, Arun H.; Jain, Ankit P.; Solanki, Hitendra S.; Radhakrishnan, Aneesha; Pinto, Sneha M.; Sahasrabuddhe, Nandini A.; Thomas, Joji K.; Mathur, Premendu P.; Nair, Bipin G.; Chang, Xiaofei; Prasad, T. S.Keshava; Sidransky, David; Gowda, Harsha; Chatterjee, Aditi.

In: OMICS A Journal of Integrative Biology, Vol. 21, No. 7, 01.07.2017, p. 390-403.

Research output: Contribution to journalArticle

Advani, J, Subbannayya, Y, Patel, K, Khan, AA, Patil, AH, Jain, AP, Solanki, HS, Radhakrishnan, A, Pinto, SM, Sahasrabuddhe, NA, Thomas, JK, Mathur, PP, Nair, BG, Chang, X, Prasad, TSK, Sidransky, D, Gowda, H & Chatterjee, A 2017, 'Long-Term Cigarette Smoke Exposure and Changes in MiRNA Expression and Proteome in Non-Small-Cell Lung Cancer', OMICS A Journal of Integrative Biology, vol. 21, no. 7, pp. 390-403. https://doi.org/10.1089/omi.2017.0045
Advani, Jayshree ; Subbannayya, Yashwanth ; Patel, Krishna ; Khan, Aafaque Ahmad ; Patil, Arun H. ; Jain, Ankit P. ; Solanki, Hitendra S. ; Radhakrishnan, Aneesha ; Pinto, Sneha M. ; Sahasrabuddhe, Nandini A. ; Thomas, Joji K. ; Mathur, Premendu P. ; Nair, Bipin G. ; Chang, Xiaofei ; Prasad, T. S.Keshava ; Sidransky, David ; Gowda, Harsha ; Chatterjee, Aditi. / Long-Term Cigarette Smoke Exposure and Changes in MiRNA Expression and Proteome in Non-Small-Cell Lung Cancer. In: OMICS A Journal of Integrative Biology. 2017 ; Vol. 21, No. 7. pp. 390-403.
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