Abstract
Long noncoding RNAs (lncRNAs) play critical roles during tumorigenesis by functioning as scaffolds that regulate protein-protein, protein-DNA or protein-RNA interactions. Using a clinically guided genetic screening approach, we identified lncRNA in nonhomologous end joining (NHEJ) pathway 1 (LINP1), which is overexpressed in human triple-negative breast cancer. We found that LINP1 enhances repair of DNA double-strand breaks by serving as a scaffold linking Ku80 and DNA-PKcs, thereby coordinating the NHEJ pathway. Importantly, blocking LINP1, which is regulated by p53 and epidermal growth factor receptor (EGFR) signaling, increases the sensitivity of the tumor-cell response to radiotherapy in breast cancer.
Original language | English (US) |
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Pages (from-to) | 522-530 |
Number of pages | 9 |
Journal | Nature Structural and Molecular Biology |
Volume | 23 |
Issue number | 6 |
DOIs | |
State | Published - Jun 7 2016 |
Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Structural Biology