Long interspersed element sequencing (L1-seq): A method to identify somatic LINE-1 insertions in the human genome

Tara T. Doucet, Haig H. Kazazian

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

L1-seq is a high-throughput sequencing technique which is utilized to identify novel L1 insertions in genomic DNA samples of interest. Using special diagnostic nucleotides unique to the youngest and most active L1 sequence, we can amplify new somatic insertions. This technique has helped to establish the number of L1 insertions present in the general population as well as the variation among individuals with regard to their complement of active L1 elements. More recently, this technique has been employed to assess the level of retrotransposition occurring in various diseases such as cancer. These efforts try to establish a connection between the process of retrotransposition and disease development and/or progression.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages79-93
Number of pages15
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1400
ISSN (Print)1064-3745

Keywords

  • L1
  • LINE-1
  • Next-generation DNA sequencing
  • Non-LTR retrotransposon
  • Retroelement

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Doucet, T. T., & Kazazian, H. H. (2016). Long interspersed element sequencing (L1-seq): A method to identify somatic LINE-1 insertions in the human genome. In Methods in Molecular Biology (pp. 79-93). (Methods in Molecular Biology; Vol. 1400). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3372-3_5