Long- but not short-term adult-onset, isolated GH deficiency in male mice leads to deterioration of β-cell function, which cannot be accounted for by changes in -βcell mass

Jose Cordoba-Chacon, Manuel D. Gahete, Naveen K. Pokala, David Geldermann, Maria Alba, Roberto Salvatori, Raul M. Luque, Rhonda D. Kineman

Research output: Contribution to journalArticle

Abstract

Developmental models of GH deficiency (GHD) and excess indicate that GH is positively associated with β-cell mass. Therefore, the reduction in GH levels observed with age and weight gain may contribute to the age-related decline in β-cell function. To test this hypothesis, β-cell mass and function were assessed in a mouse model of adult-onset, isolated GHD (AOiGHD). β-Cell mass did not differ between low-fat (LF)-fed AOiGHD and controls. However, high fat-fed AOiGHD mice displayed impaired expansion of β-cell mass and a reduction of bromodeoxyuridine-labeled islet cells, whereas in vitro β-cell function (basal and glucose-stimulated insulin secretion [GSIS]) did not differ from controls. In contrast, duration of AOiGHD differentially altered in vitro β-cell function in LF-fed mice. Specifically, islets from young LF-fed AOiGHD mice showed significant reductions in insulin content and basal insulin secretion, but GSIS was similar to that of controls. A similar islet phenotype was observed in a developmental model of isolated GHD (GH-releasing hormone knockout). Given that LF- and high fat-fed AOiGHD mice, as well as GH-releasing hormone knockout mice, display improved insulin sensitivity, islet changes may be due to reduced insulin demand, rather than primary β-cell dysfunction. However, islets from older LF-fed AOiGHD mice exhibited impaired GSIS, associated with reduced expression of genes important to maintain glucose sensing, suggesting that factors secondary to AOiGHD can alter β-cell function with age. AOiGHD mice exhibited postprandial hypertriglyceridemia and increased pancreatic expression of lipid/inflammatory stress response genes (activating transcription factor 3 and peroxisome proliferator activator receptor β/δ). Therefore, we speculate that these changes may initially protect the AOiGHD β-cell, but with age, lipotoxicity may impair β-cell function.

Original languageEnglish (US)
Pages (from-to)726-735
Number of pages10
JournalEndocrinology
Volume155
Issue number3
DOIs
StatePublished - Mar 2014

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Pituitary Dwarfism
Fats
Insulin
Glucose
Activating Transcription Factor 3
Hormones
Peroxisome Proliferators
Hypertriglyceridemia
Bromodeoxyuridine
Islets of Langerhans
Knockout Mice
Weight Gain
Insulin Resistance

ASJC Scopus subject areas

  • Endocrinology

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Long- but not short-term adult-onset, isolated GH deficiency in male mice leads to deterioration of β-cell function, which cannot be accounted for by changes in -βcell mass. / Cordoba-Chacon, Jose; Gahete, Manuel D.; Pokala, Naveen K.; Geldermann, David; Alba, Maria; Salvatori, Roberto; Luque, Raul M.; Kineman, Rhonda D.

In: Endocrinology, Vol. 155, No. 3, 03.2014, p. 726-735.

Research output: Contribution to journalArticle

Cordoba-Chacon, Jose ; Gahete, Manuel D. ; Pokala, Naveen K. ; Geldermann, David ; Alba, Maria ; Salvatori, Roberto ; Luque, Raul M. ; Kineman, Rhonda D. / Long- but not short-term adult-onset, isolated GH deficiency in male mice leads to deterioration of β-cell function, which cannot be accounted for by changes in -βcell mass. In: Endocrinology. 2014 ; Vol. 155, No. 3. pp. 726-735.
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