Localizer: Fast, accurate, open-source, and modular software package for superresolution microscopy

Peter Dedecker, Sam Duwé, Robert K. Neely, Jin Zhang

Research output: Contribution to journalArticle

Abstract

We present Localizer, a freely available and open source software package that implements the computational data processing inherent to several types of superresolution fluorescence imaging, such as localization (PALM/STORM/GSDIM) and fluctuation imaging (SOFI/pcSOFI). Localizer delivers high accuracy and performance and comes with a fully featured and easy-to-use graphical user interface but is also designed to be integrated in higher-level analysis environments. Due to its modular design, Localizer can be readily extended with new algorithms as they become available, while maintaining the same interface and performance. We provide front-ends for running Localizer from Igor Pro, Matlab, or as a stand-alone program. We show that Localizer performs favorably when compared with two existing superresolution packages, and to our knowledge is the only freely available implementation of SOFI/pcSOFI microscopy. By dramatically improving the analysis performance and ensuring the easy addition of current and future enhancements, Localizer strongly improves the usability of superresolution imaging in a variety of biomedical studies.

Original languageEnglish (US)
Article number126008
JournalJournal of Biomedical Optics
Volume17
Issue number12
DOIs
StatePublished - Dec 2012

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Software packages
Microscopic examination
microscopy
computer programs
Imaging techniques
graphical user interface
Graphical user interfaces
Fluorescence
fluorescence
augmentation
Open source software

Keywords

  • Image processing
  • Subdiffraction limit

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Localizer : Fast, accurate, open-source, and modular software package for superresolution microscopy. / Dedecker, Peter; Duwé, Sam; Neely, Robert K.; Zhang, Jin.

In: Journal of Biomedical Optics, Vol. 17, No. 12, 126008, 12.2012.

Research output: Contribution to journalArticle

Dedecker, Peter ; Duwé, Sam ; Neely, Robert K. ; Zhang, Jin. / Localizer : Fast, accurate, open-source, and modular software package for superresolution microscopy. In: Journal of Biomedical Optics. 2012 ; Vol. 17, No. 12.
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