TY - JOUR
T1 - Localized diffusion magnetic resonance micro-imaging of the live mouse brain
AU - Wu, Dan
AU - Reisinger, Dominik
AU - Xu, Jiadi
AU - Fatemi, S. Ali
AU - van Zijl, Peter C.M.
AU - Mori, Susumu
AU - Zhang, Jiangyang
N1 - Funding Information:
This work was supported by NIH grants R01NS070909 (J.Z.), R01HD074593 (J.Z.), R01AG20012 (S.M.), R01EB003543 (S.M.), 1S10 RR028955 (P.V.Z.), and P41EB015909 (P.V.Z.). The authors thank Mary McAllister (Department of Radiology, Johns Hopkins University School of Medicine) for her editorial assistance.
PY - 2014/5/1
Y1 - 2014/5/1
N2 - High-resolution diffusion MRI (dMRI) is useful for resolving complex microstructures in the mouse brain, but technically challenging for in vivo studies due to the long scan time. In this study, selective excitation and a three-dimensional fast imaging sequence were used to achieve in vivo high-resolution dMRI of the mouse brain at 11.7. Tesla. By reducing the field of view using spatially selective radio frequency pulses, we were able to focus on targeted brain structures and acquire high angular resolution diffusion imaging (HARDI) data at an isotropic resolution of 0.1. mm and 30 diffusion encoding directions in approximately 1. h. We investigated the complex tissue microstructures of the mouse hippocampus, cerebellum, and several cortical areas using this localized dMRI approach, and compared the results with histological sections stained with several axonal and dendritic markers. In the mouse visual cortex, the results showed predominately radially arranged structures in an outer layer and tangentially arranged structures in an inner layer, similar to observations from postmortem human brain specimens.
AB - High-resolution diffusion MRI (dMRI) is useful for resolving complex microstructures in the mouse brain, but technically challenging for in vivo studies due to the long scan time. In this study, selective excitation and a three-dimensional fast imaging sequence were used to achieve in vivo high-resolution dMRI of the mouse brain at 11.7. Tesla. By reducing the field of view using spatially selective radio frequency pulses, we were able to focus on targeted brain structures and acquire high angular resolution diffusion imaging (HARDI) data at an isotropic resolution of 0.1. mm and 30 diffusion encoding directions in approximately 1. h. We investigated the complex tissue microstructures of the mouse hippocampus, cerebellum, and several cortical areas using this localized dMRI approach, and compared the results with histological sections stained with several axonal and dendritic markers. In the mouse visual cortex, the results showed predominately radially arranged structures in an outer layer and tangentially arranged structures in an inner layer, similar to observations from postmortem human brain specimens.
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U2 - 10.1016/j.neuroimage.2014.01.014
DO - 10.1016/j.neuroimage.2014.01.014
M3 - Article
C2 - 24440780
AN - SCOPUS:84894251502
SN - 1053-8119
VL - 91
SP - 12
EP - 20
JO - NeuroImage
JF - NeuroImage
ER -