Localization, regulation, and substrate transport properties of Bpt1p, a Saccharomyces cerevisiae MRP-type ABC transporter

Kailash Gulshan Sharma, Deborah L. Mason, Guosheng Liu, Philip A. Rea, Anand K. Bachhawat, Susan Doris Michaelis

Research output: Contribution to journalArticle

Abstract

Saccharomyces cerevisiae Bpt1p is an ATP-binding cassette (ABC) protein that belongs to the MRP subfamily and is a close homologue of the glutathione conjugate (GS conjugate) transporter Ycf1p. The function of Bpt1p has previously been evaluated only in vitro, by using nonphysiological substrates. In the present study we examined the localization, regulation, and transport properties of Bpt1p in vivo, as well as its capacity to transport a set of prototypical MRP substrates in vitro. Our results show that Bpt1p, like Ycf1p, localizes to the yeast vacuolar membrane, plays a role in cadmium detoxification and ade2 pigmentation in vivo, and can participate in the transport of GS conjugates and glucuronate conjugates, as well as free glutathione, in vitro. However, in all of these cases the contribution of Bpt1p is substantially less than that of Ycf1p. In addition, the expression patterns of YCF1 and BPT1 differ significantly. Whereas YCF1 expression is markedly increased by cadmium, adenine limitation in an ade2 strain, or overexpression of the stress-responsive transcription factor Yap1p, BPT1 expression is only modestly affected under these conditions. Thus, although the functional capabilities of Bpt1p and Ycf1p overlap, their differences in regulation and substrate preference imply that they contribute to cellular detoxification processes in different ways.

Original languageEnglish (US)
Pages (from-to)391-400
Number of pages10
JournalEukaryotic Cell
Volume1
Issue number3
DOIs
StatePublished - Jun 2002

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ABC transporters
ATP-Binding Cassette Transporters
Saccharomyces cerevisiae
glutathione
cadmium
Cadmium
Glutathione
adenine
pigmentation
transporters
Glucuronic Acid
transcription factors
Pigmentation
Adenine
yeasts
Carrier Proteins
Transcription Factors
Adenosine Triphosphate
Yeasts
Membranes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Microbiology

Cite this

Localization, regulation, and substrate transport properties of Bpt1p, a Saccharomyces cerevisiae MRP-type ABC transporter. / Sharma, Kailash Gulshan; Mason, Deborah L.; Liu, Guosheng; Rea, Philip A.; Bachhawat, Anand K.; Michaelis, Susan Doris.

In: Eukaryotic Cell, Vol. 1, No. 3, 06.2002, p. 391-400.

Research output: Contribution to journalArticle

Sharma, Kailash Gulshan ; Mason, Deborah L. ; Liu, Guosheng ; Rea, Philip A. ; Bachhawat, Anand K. ; Michaelis, Susan Doris. / Localization, regulation, and substrate transport properties of Bpt1p, a Saccharomyces cerevisiae MRP-type ABC transporter. In: Eukaryotic Cell. 2002 ; Vol. 1, No. 3. pp. 391-400.
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