Localization of vascular endothelial growth factor in human retina and choroid

Gerard Anthony Lutty, D. Scott McLeod, Carol Merges, Annia Diggs, Jean Plouét

Research output: Contribution to journalArticle

Abstract

Objective: To examine the distribution and relative levels of vascular endothelial growth factor (VEGF) in the nondiabetic and preproliferative diabetic human retina and choroid. Methods: Immunohistochemical localization was performed on frozen sections from cryopreserved postmortem human tissue using a polyclonal antibody against VEGF and a streptavidin peroxidase system. Eyes from 5 subjects without diabetes and 8 subjects with diabetes were examined and analyzed using a 7-point immunohistochemical grading system. Results: In subjects without diabetes, weak or no VEGF immunoreactivity was associated with retinal blood vessels. In subjects with diabetes, we found significantly increased immunoreactivity in the retinal vascular endothelium and blood vessel walls. Vascular endothelial growth factor immunoreactivity was also associated with intravascular leukocytes in subjects with and without diabetes. In the choroid of subjects without diabetes, immunoreactivity was almost exclusively associated with intravascular leukocytes, whereas in diabetic subjects, immunoreactivity was localized within choriocapillaris endothelium, choroidal neovascular endothelium, and migrating retinal pigment epithelium cells. Conclusions: The observed increase in VEGF immunoreactivity in the diabetic retina and choroid suggests that VEGF may contribute to 2 well-documented events during retinopathy: increased vascular permeability and angiogenesis.

Original languageEnglish (US)
Pages (from-to)971-977
Number of pages7
JournalArchives of Ophthalmology
Volume114
Issue number8
StatePublished - Aug 1996

Fingerprint

Choroid
Vascular Endothelial Growth Factor A
Retina
Retinal Vessels
Endothelium
Leukocytes
Streptavidin
Retinal Pigment Epithelium
Vascular Endothelium
Frozen Sections
Capillary Permeability
Peroxidase
Blood Vessels
human VEGFA protein
Antibodies

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Lutty, G. A., McLeod, D. S., Merges, C., Diggs, A., & Plouét, J. (1996). Localization of vascular endothelial growth factor in human retina and choroid. Archives of Ophthalmology, 114(8), 971-977.

Localization of vascular endothelial growth factor in human retina and choroid. / Lutty, Gerard Anthony; McLeod, D. Scott; Merges, Carol; Diggs, Annia; Plouét, Jean.

In: Archives of Ophthalmology, Vol. 114, No. 8, 08.1996, p. 971-977.

Research output: Contribution to journalArticle

Lutty, GA, McLeod, DS, Merges, C, Diggs, A & Plouét, J 1996, 'Localization of vascular endothelial growth factor in human retina and choroid', Archives of Ophthalmology, vol. 114, no. 8, pp. 971-977.
Lutty, Gerard Anthony ; McLeod, D. Scott ; Merges, Carol ; Diggs, Annia ; Plouét, Jean. / Localization of vascular endothelial growth factor in human retina and choroid. In: Archives of Ophthalmology. 1996 ; Vol. 114, No. 8. pp. 971-977.
@article{7c90cacfec0448e680cf9b46d23e6614,
title = "Localization of vascular endothelial growth factor in human retina and choroid",
abstract = "Objective: To examine the distribution and relative levels of vascular endothelial growth factor (VEGF) in the nondiabetic and preproliferative diabetic human retina and choroid. Methods: Immunohistochemical localization was performed on frozen sections from cryopreserved postmortem human tissue using a polyclonal antibody against VEGF and a streptavidin peroxidase system. Eyes from 5 subjects without diabetes and 8 subjects with diabetes were examined and analyzed using a 7-point immunohistochemical grading system. Results: In subjects without diabetes, weak or no VEGF immunoreactivity was associated with retinal blood vessels. In subjects with diabetes, we found significantly increased immunoreactivity in the retinal vascular endothelium and blood vessel walls. Vascular endothelial growth factor immunoreactivity was also associated with intravascular leukocytes in subjects with and without diabetes. In the choroid of subjects without diabetes, immunoreactivity was almost exclusively associated with intravascular leukocytes, whereas in diabetic subjects, immunoreactivity was localized within choriocapillaris endothelium, choroidal neovascular endothelium, and migrating retinal pigment epithelium cells. Conclusions: The observed increase in VEGF immunoreactivity in the diabetic retina and choroid suggests that VEGF may contribute to 2 well-documented events during retinopathy: increased vascular permeability and angiogenesis.",
author = "Lutty, {Gerard Anthony} and McLeod, {D. Scott} and Carol Merges and Annia Diggs and Jean Plou{\'e}t",
year = "1996",
month = "8",
language = "English (US)",
volume = "114",
pages = "971--977",
journal = "JAMA Ophthalmology",
issn = "2168-6165",
publisher = "American Medical Association",
number = "8",

}

TY - JOUR

T1 - Localization of vascular endothelial growth factor in human retina and choroid

AU - Lutty, Gerard Anthony

AU - McLeod, D. Scott

AU - Merges, Carol

AU - Diggs, Annia

AU - Plouét, Jean

PY - 1996/8

Y1 - 1996/8

N2 - Objective: To examine the distribution and relative levels of vascular endothelial growth factor (VEGF) in the nondiabetic and preproliferative diabetic human retina and choroid. Methods: Immunohistochemical localization was performed on frozen sections from cryopreserved postmortem human tissue using a polyclonal antibody against VEGF and a streptavidin peroxidase system. Eyes from 5 subjects without diabetes and 8 subjects with diabetes were examined and analyzed using a 7-point immunohistochemical grading system. Results: In subjects without diabetes, weak or no VEGF immunoreactivity was associated with retinal blood vessels. In subjects with diabetes, we found significantly increased immunoreactivity in the retinal vascular endothelium and blood vessel walls. Vascular endothelial growth factor immunoreactivity was also associated with intravascular leukocytes in subjects with and without diabetes. In the choroid of subjects without diabetes, immunoreactivity was almost exclusively associated with intravascular leukocytes, whereas in diabetic subjects, immunoreactivity was localized within choriocapillaris endothelium, choroidal neovascular endothelium, and migrating retinal pigment epithelium cells. Conclusions: The observed increase in VEGF immunoreactivity in the diabetic retina and choroid suggests that VEGF may contribute to 2 well-documented events during retinopathy: increased vascular permeability and angiogenesis.

AB - Objective: To examine the distribution and relative levels of vascular endothelial growth factor (VEGF) in the nondiabetic and preproliferative diabetic human retina and choroid. Methods: Immunohistochemical localization was performed on frozen sections from cryopreserved postmortem human tissue using a polyclonal antibody against VEGF and a streptavidin peroxidase system. Eyes from 5 subjects without diabetes and 8 subjects with diabetes were examined and analyzed using a 7-point immunohistochemical grading system. Results: In subjects without diabetes, weak or no VEGF immunoreactivity was associated with retinal blood vessels. In subjects with diabetes, we found significantly increased immunoreactivity in the retinal vascular endothelium and blood vessel walls. Vascular endothelial growth factor immunoreactivity was also associated with intravascular leukocytes in subjects with and without diabetes. In the choroid of subjects without diabetes, immunoreactivity was almost exclusively associated with intravascular leukocytes, whereas in diabetic subjects, immunoreactivity was localized within choriocapillaris endothelium, choroidal neovascular endothelium, and migrating retinal pigment epithelium cells. Conclusions: The observed increase in VEGF immunoreactivity in the diabetic retina and choroid suggests that VEGF may contribute to 2 well-documented events during retinopathy: increased vascular permeability and angiogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0029739621&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029739621&partnerID=8YFLogxK

M3 - Article

VL - 114

SP - 971

EP - 977

JO - JAMA Ophthalmology

JF - JAMA Ophthalmology

SN - 2168-6165

IS - 8

ER -