Localization of the Locus Coeruleus in the Mouse Brain

Katharina Schmidt, Bilal Bari, Martina Ralle, Clorissa Washington-Hughes, Abigael Muchenditsi, Evan Maxey, Svetlana Lutsenko

Research output: Contribution to journalArticle

Abstract

The locus coeruleus (LC) is a major hub of norepinephrine producing neurons that modulate a number of physiological functions. Structural or functional abnormalities of LC impact several brain regions including cortex, hippocampus, and cerebellum and may contribute to depression, bipolar disorder, anxiety, as well as Parkinson disease and Alzheimer disease. These disorders are often associated with metal misbalance, but the role of metals in LC is only partially understood. Morphologic and functional studies of LC are needed to better understand the human pathologies and contribution of metals. Mice are a widely used experimental model, but the mouse LC is small (~0.3 mm diameter) and hard to identify for a non-expert. Here, we describe a step-by-step immunohistochemistry-based protocol to localize the LC in the mouse brain. Dopamine-β-hydroxylase (DBH), and alternatively, tyrosine hydroxylase (TH), both enzymes highly expressed in the LC, are used as immunohistochemical markers in brain slices. Sections adjacent to LC-containing sections can be used for further analysis, including histology for morphological studies, metabolic testing, as well as metal imaging by X-ray fluorescence microscopy (XFM).

Original languageEnglish (US)
JournalJournal of visualized experiments : JoVE
Issue number145
DOIs
StatePublished - Mar 7 2019

Fingerprint

Locus Coeruleus
Brain
Metals
Metal testing
Norepinephrine
Histology
Fluorescence microscopy
Tyrosine 3-Monooxygenase
Pathology
Mixed Function Oxygenases
Neurons
Dopamine
Enzymes
Imaging techniques
X rays
Bipolar Disorder
Fluorescence Microscopy
Cerebellum
Parkinson Disease
Hippocampus

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Schmidt, K., Bari, B., Ralle, M., Washington-Hughes, C., Muchenditsi, A., Maxey, E., & Lutsenko, S. (2019). Localization of the Locus Coeruleus in the Mouse Brain. Journal of visualized experiments : JoVE, (145). https://doi.org/10.3791/58652

Localization of the Locus Coeruleus in the Mouse Brain. / Schmidt, Katharina; Bari, Bilal; Ralle, Martina; Washington-Hughes, Clorissa; Muchenditsi, Abigael; Maxey, Evan; Lutsenko, Svetlana.

In: Journal of visualized experiments : JoVE, No. 145, 07.03.2019.

Research output: Contribution to journalArticle

Schmidt, K, Bari, B, Ralle, M, Washington-Hughes, C, Muchenditsi, A, Maxey, E & Lutsenko, S 2019, 'Localization of the Locus Coeruleus in the Mouse Brain', Journal of visualized experiments : JoVE, no. 145. https://doi.org/10.3791/58652
Schmidt K, Bari B, Ralle M, Washington-Hughes C, Muchenditsi A, Maxey E et al. Localization of the Locus Coeruleus in the Mouse Brain. Journal of visualized experiments : JoVE. 2019 Mar 7;(145). https://doi.org/10.3791/58652
Schmidt, Katharina ; Bari, Bilal ; Ralle, Martina ; Washington-Hughes, Clorissa ; Muchenditsi, Abigael ; Maxey, Evan ; Lutsenko, Svetlana. / Localization of the Locus Coeruleus in the Mouse Brain. In: Journal of visualized experiments : JoVE. 2019 ; No. 145.
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