Localization of Sequences Required in cis for Yeast Ty1 Element Transposition near the Long Terminal Repeats

Analysis of Mini-Ty1 Elements

Hua Hu, Jef D. Boeke

Research output: Contribution to journalArticle

Abstract

In order to identify and characterize sequences within Ty1 elements which are required in as for transposition, a series of mini-Ty1 plasmids were constructed and tested for transposition. Mini-Ty1s are deletion mutants of the Ty1-H3 element; Ty1 gene products required for transposition are supplied in trans from a helper Ty1 which has intact open reading frames but lacks a 3′ long terminal repeat (LTR) and therefore cannot transpose itself. Up to 5 kilobase pairs of internal sequences of the 6-kilobase-pair-long Ty1 element can be deleted without a significant effect on transposition. The smallest mini-Ty1 element capable of transposition contains the 3′ LTR and the transcribed portion of the 5′ LTR, 285 base pairs (bp) of internal sequence 3′ to the 5′ LTR, and 23 bp of internal sequence 5′ to the 3′ LTR. We conclude that Ty1-encoded proteins can act in trans and that cis-acting sequences in Ty1-H3 are all within or near the LTRs. Further deletion of the 285-bp internal sequence adjacent to the 5′ LTR significantly reduced transposition frequency, and the mini-Ty1 RNA produced failed to be packaged into the viruslike particles efficiently. Surprisingly, several nonhomologous cellular mRNAs were also associated with viruslike particles.

Original languageEnglish (US)
Pages (from-to)2695-2702
Number of pages8
JournalMolecular and Cellular Biology
Volume10
Issue number6
StatePublished - Jun 1990

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Terminal Repeat Sequences
Yeasts
Base Pairing
Open Reading Frames
Plasmids
RNA
Messenger RNA
Genes
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Localization of Sequences Required in cis for Yeast Ty1 Element Transposition near the Long Terminal Repeats : Analysis of Mini-Ty1 Elements. / Hu, Hua; Boeke, Jef D.

In: Molecular and Cellular Biology, Vol. 10, No. 6, 06.1990, p. 2695-2702.

Research output: Contribution to journalArticle

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