Localization of phosphatidylinositol signaling components in rat taste cells

Role in bitter taste transduction

Paul M. Hwang, Ajay Verma, David S. Bredt, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

To assess the role of phosphatidylinositol turnover in taste transduction we have visualized, in rat tongue, ATP-dependent endoplasmic reticular accumulation of 45Ca2+, inositol 1,4,5-trisphosphate receptor binding sites, and phosphatidylinositol turnover monitored by autoradiography of [3H]cytidine diphosphate diacylglycerol formed from [3H]cytidine. Accumulated 45Ca2+, inositol 1,4,5-trisphosphate receptors, and phosphatidylinositol turnover are selectively localized to apical areas of the taste buds of circumvallate papillae, which are associated with bitter taste. Further evidence for a role of phosphatidylinositol turnover in bitter taste is our observation of a rapid, selective increase in mass levels of inositol 1,4,5-trisphosphate elicited by low concentrations of denatonium, a potently bitter tastant.

Original languageEnglish (US)
Pages (from-to)7395-7399
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number19
StatePublished - 1990

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Phosphatidylinositols
Inositol 1,4,5-Trisphosphate Receptors
Cytidine Diphosphate
Taste Buds
Cytidine
Inositol 1,4,5-Trisphosphate
Diglycerides
Autoradiography
Tongue
Adenosine Triphosphate
Binding Sites
Observation

Keywords

  • Circumvallate papillae
  • Cytidine diphosphate diacylglycerol
  • Denatonium
  • Inositol 1,4,5-trisphosphate
  • Von Ebner's gland

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Localization of phosphatidylinositol signaling components in rat taste cells : Role in bitter taste transduction. / Hwang, Paul M.; Verma, Ajay; Bredt, David S.; Snyder, Solomon H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 19, 1990, p. 7395-7399.

Research output: Contribution to journalArticle

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AU - Snyder, Solomon H

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