Localization of oligomycin-sensitive ADP-ATP exchange activity in rat liver mitochondria

Carl A. Schnaitman, Peter L Pedersen

Research output: Contribution to journalArticle

Abstract

Intact rat liver mitochondria catalyze an ADP-ATP exchange which is partially inhibited by oligomycin and dinitrophenol (Wadkins and Lehninger, 1963; Guillory and Slater, 1965). It has been postulated that the dinitrophenol- and oligomycin-sensitive ADP-ATP exchange reaction is catalyzed by the enzyme(s) participating in ATP formation during oxidative phosphorylation (Wadkins and Lehninger, 1958; 1963). However, the presence of large amounts of adenylate kinase and nucleoside diphosphokinase, which catalyze oligomycin-insensitive ADP-ATP exchanges, has rendered further investigation of the oligomycin-sensitive exchange difficult. In this communication it is shown that when rat liver mitochondria are fractionated with digitonin the oligomycin-sensitive ADP-ATP exchange activity is recovered with the inner membrane-matrix fraction, while all of the nucleoside diphosphokinase and adenylate kinase activity is released with the outer membrane. The exchange activity found in the inner membrane-matrix fraction is localized in the inner membrane and is completely inhibited by oligomycin, dinitrophenol, and atractyloside when assayed in the absence of added Mg++. Thus, the oligomycin-sensitive ADP-ATP exchange activity observed in intact mitochondria does not appear to be catalyzed by nucleoside diphosphokinase or adenylate kinase.

Original languageEnglish (US)
Pages (from-to)428-433
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume30
Issue number4
StatePublished - Feb 26 1968

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Oligomycins
Mitochondria
Liver Mitochondrion
Liver
Adenosine Diphosphate
Rats
Adenosine Triphosphate
nucleoside phosphotransferase
Nucleoside-Diphosphate Kinase
Dinitrophenols
Adenylate Kinase
Membranes
Atractyloside
Digitonin
Oxidative Phosphorylation
Communication
Enzymes

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Localization of oligomycin-sensitive ADP-ATP exchange activity in rat liver mitochondria. / Schnaitman, Carl A.; Pedersen, Peter L.

In: Biochemical and Biophysical Research Communications, Vol. 30, No. 4, 26.02.1968, p. 428-433.

Research output: Contribution to journalArticle

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