Localization of mRNA for three distinct α1-adrenergic receptor subtypes in human tissues: Implications for human α-adrenergic physiology

David T. Price, Robert J. Lefkowitz, Marc G. Caron, Dan Berkowitz, Debra A. Schwinn

Research output: Contribution to journalArticlepeer-review

Abstract

α1-Adrenergic receptors (α1ARs) are virtually ubiquitous in human tissues and mediate important physiological functions as diverse as smooth muscle contraction, glycogenolysis, and myocardial inotropy. At least three α1AR subtypes (α(1A/D), α(1B), and α(1C)) have been described using molecular and pharmacological techniques. The identification of species heterogeneity (rat versus rabbit) in α1AR subtype distribution has made it imperative to determine the distribution of α1AR subtypes in human tissues. Accordingly, RNA extracted from human tissues was analyzed using RNase protection assays to determine α1AR subtype expression. Of the cloned α1ARs, α(1C)AR mRNA predominates in many human tissues (heart, liver, cerebellum, and cerebral cortex), in contrast to its restricted distribution in both rats and rabbits. α(1B)AR mRNA is present in highest concentrations in human spleen, kidney, and fetal brain. α(1A/D)AR mRNA is present in highest concentrations in human aorta and cerebral cortex. Hence, α1AR subtype mRNA distribution is tissue selective and differs from that reported for rats and rabbits. These results have potentially significant implications for understanding human adrenergic physiology and are important for the rational development of α1AR subtype-selective drugs.

Original languageEnglish (US)
Pages (from-to)171-175
Number of pages5
JournalMolecular Pharmacology
Volume45
Issue number2
StatePublished - Feb 1994

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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