Localization of glucose-6-phosphate dehydrogenase in mouse and man by in situ hybridization: Evidence for a single locus and transposition of homologous X-linked genes

P. A. Martin-DeLeon, S. F. Wolf, G. Persico, D. Toniolo, G. Martini, Barbara R Migeon

Research output: Contribution to journalArticle

Abstract

By hybridizing a tritiatcd human genomic probe (pGD3) to metaphase chromosomes in situ, we have localized the gene for glucose-6-phosphate dehydrogenase (G6PD) in both the human and mouse complement. The locus on the intact human X chromosome is close to the telomere on the long arm. confirming the assignment based on studies of an X/autosome translocation in human-mouse hybrids. Although the signahbackground ratio was reduced for the heterologous hybridization of the human probe to mouse metaphases, 20% of the grains were on the X chromosome and 93% of these were in the A region, relatively close to the centromere. The location of G6PD in mouse and man reflects inirachromosomal transposition of these homologous X loci. Genomic DNAs from mouse and man and from hybrids with human X/autosome translocations were digested with several restriction enzymes including EcoRl, Pstl, and Hpcill, and Southern blots were probed with 32P-pGD3. The results of the analysis also confirm the human G6PD assignment and are consistent with a single copy of the locus in the haploid genome of both species.

Original languageEnglish (US)
Pages (from-to)87-92
Number of pages6
JournalCytogenetic and Genome Research
Volume39
Issue number2
DOIs
StatePublished - 1985

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X-Linked Genes
Glucosephosphate Dehydrogenase
In Situ Hybridization
Metaphase
Chromosomes, Human, X
Centromere
Haploidy
Telomere
X Chromosome
Southern Blotting
Chromosomes
Genome
DNA
Enzymes
Genes

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Genetics(clinical)
  • Cell Biology

Cite this

Localization of glucose-6-phosphate dehydrogenase in mouse and man by in situ hybridization : Evidence for a single locus and transposition of homologous X-linked genes. / Martin-DeLeon, P. A.; Wolf, S. F.; Persico, G.; Toniolo, D.; Martini, G.; Migeon, Barbara R.

In: Cytogenetic and Genome Research, Vol. 39, No. 2, 1985, p. 87-92.

Research output: Contribution to journalArticle

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