Localization of G6PD and HPRT to different arms of the X chromosome of the North American marsupial (Didelphis virginiana) by in situ hybridization and delection mapping: Evolutionary significance

Daniel J. Driscoll; Barbara R. Migeon

doi:10.1016/0888-7543(88)90121-8

Localization of G6PD and HPRT to different arms of the X chromosome of the North American marsupial (Didelphis virginiana) by in situ hybridization and delection mapping: Evolutionary significance

Daniel J. Driscoll, Barbara R. Migeon

School of Medicine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We have mapped HPRT and G6PD loci on the X chromosome in the American opossum, Didelphis virginiana, by in situ hybridization to cells derived from two females by using genomic opossum DNA as probes. The localizations (G6PD to Xp13 and HPRT to Xq21), indicating that the two genes are separated by the centromere, were confirmed by results of hybridization to X chromosomes with deletions that include the HPRT locus and opossum-mouse cell hybrids containing the relevant fragment of the opossum X chromosome.

Original language	English (US)
Pages (from-to)	308-314
Number of pages	7
Journal	Genomics
Volume	3
Issue number	4
DOIs	https://doi.org/10.1016/0888-7543(88)90121-8
State	Published - Nov 1988

ASJC Scopus subject areas

Genetics

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@article{cee37c5d81874c98ad00ae52595d06d0,

title = "Localization of G6PD and HPRT to different arms of the X chromosome of the North American marsupial (Didelphis virginiana) by in situ hybridization and delection mapping: Evolutionary significance",

abstract = "We have mapped HPRT and G6PD loci on the X chromosome in the American opossum, Didelphis virginiana, by in situ hybridization to cells derived from two females by using genomic opossum DNA as probes. The localizations (G6PD to Xp13 and HPRT to Xq21), indicating that the two genes are separated by the centromere, were confirmed by results of hybridization to X chromosomes with deletions that include the HPRT locus and opossum-mouse cell hybrids containing the relevant fragment of the opossum X chromosome.",

author = "Driscoll, {Daniel J.} and Migeon, {Barbara R.}",

note = "Funding Information: This work was supported by NIH Grant HD05465. D.J.D. is supported by NIH Postdoctoral Training Grant GM07471. We are grateful to Joyce Axelman for providing cell lines, to Suzanne Jan de Beur for DNA studies, and to Joanna Strayer for contributions to the illustrations.",

year = "1988",

month = nov,

doi = "10.1016/0888-7543(88)90121-8",

language = "English (US)",

volume = "3",

pages = "308--314",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

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TY - JOUR

T1 - Localization of G6PD and HPRT to different arms of the X chromosome of the North American marsupial (Didelphis virginiana) by in situ hybridization and delection mapping

T2 - Evolutionary significance

AU - Driscoll, Daniel J.

AU - Migeon, Barbara R.

N1 - Funding Information: This work was supported by NIH Grant HD05465. D.J.D. is supported by NIH Postdoctoral Training Grant GM07471. We are grateful to Joyce Axelman for providing cell lines, to Suzanne Jan de Beur for DNA studies, and to Joanna Strayer for contributions to the illustrations.

PY - 1988/11

Y1 - 1988/11

N2 - We have mapped HPRT and G6PD loci on the X chromosome in the American opossum, Didelphis virginiana, by in situ hybridization to cells derived from two females by using genomic opossum DNA as probes. The localizations (G6PD to Xp13 and HPRT to Xq21), indicating that the two genes are separated by the centromere, were confirmed by results of hybridization to X chromosomes with deletions that include the HPRT locus and opossum-mouse cell hybrids containing the relevant fragment of the opossum X chromosome.

AB - We have mapped HPRT and G6PD loci on the X chromosome in the American opossum, Didelphis virginiana, by in situ hybridization to cells derived from two females by using genomic opossum DNA as probes. The localizations (G6PD to Xp13 and HPRT to Xq21), indicating that the two genes are separated by the centromere, were confirmed by results of hybridization to X chromosomes with deletions that include the HPRT locus and opossum-mouse cell hybrids containing the relevant fragment of the opossum X chromosome.

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Localization of G6PD and HPRT to different arms of the X chromosome of the North American marsupial (Didelphis virginiana) by in situ hybridization and delection mapping: Evolutionary significance

Abstract

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