Localization of epidermal growth factor receptors and putative neuoblasts in human subependymal zone

Cynthia Shannon Weickert, Maree J. Webster, Sarah M. Colvin, Mary M. Herman, Thomas Hyde, Daniel Weinberger, Joel Kleinman

Research output: Contribution to journalArticle

Abstract

Studies in rodents and monkeys suggest that neuronal precursor cells continue to exist and differentiate well into adulthood in these species. These results challenge the long held assumption that neurogenesis does not occur in the postnatal human brain. We examined the rostral subependymal zone (SEZ) of postnatal human brain for expression of cell phenotypic markers that have been associated with neuronal precursors and neuroblasts in rodent brain. We found epidermal growth factor receptor (EGF-R) mRNA and protein to be expressed in infant, teen, young adult, and adult human SEZ. Some SEZ cells expressed the polysialic acid form of neural cell adhesion molecule (PSA-NCAM), characteristic of migrating neuroblasts, as well as class III β- tubulin and Hu protein, characteristic of neuroblasts and early neurons. These neuroblast-like cells were negative for glial fibrillary acidic protein (GFAP), 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNPase), and vimentin, suggesting that they were not differentiating as glia. Our results show that neuroblast-like cells exist in the human SEZ and support the theory that SEZ of postnatal human brain has neurogenic potential.

Original languageEnglish (US)
Pages (from-to)359-372
Number of pages14
JournalJournal of Comparative Neurology
Volume423
Issue number3
DOIs
StatePublished - Jul 31 2000
Externally publishedYes

Fingerprint

nucleotidase
Brain
Rodentia
Neural Cell Adhesion Molecules
Cyclic Nucleotides
Glial Fibrillary Acidic Protein
Neurogenesis
Vimentin
Tubulin
Epidermal Growth Factor Receptor
Neuroglia
Haplorhini
Young Adult
Proteins
ErbB Receptors
Neurons
Messenger RNA
polysialic acid

Keywords

  • Class III β-tubulin
  • CNPase
  • Hu
  • Neu N
  • Neurogenesis
  • Precursor
  • PSA-NCAM
  • Subventricular zone

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Localization of epidermal growth factor receptors and putative neuoblasts in human subependymal zone. / Weickert, Cynthia Shannon; Webster, Maree J.; Colvin, Sarah M.; Herman, Mary M.; Hyde, Thomas; Weinberger, Daniel; Kleinman, Joel.

In: Journal of Comparative Neurology, Vol. 423, No. 3, 31.07.2000, p. 359-372.

Research output: Contribution to journalArticle

Weickert, Cynthia Shannon ; Webster, Maree J. ; Colvin, Sarah M. ; Herman, Mary M. ; Hyde, Thomas ; Weinberger, Daniel ; Kleinman, Joel. / Localization of epidermal growth factor receptors and putative neuoblasts in human subependymal zone. In: Journal of Comparative Neurology. 2000 ; Vol. 423, No. 3. pp. 359-372.
@article{d30cb67dc3874079a75d25ca7c6d5aef,
title = "Localization of epidermal growth factor receptors and putative neuoblasts in human subependymal zone",
abstract = "Studies in rodents and monkeys suggest that neuronal precursor cells continue to exist and differentiate well into adulthood in these species. These results challenge the long held assumption that neurogenesis does not occur in the postnatal human brain. We examined the rostral subependymal zone (SEZ) of postnatal human brain for expression of cell phenotypic markers that have been associated with neuronal precursors and neuroblasts in rodent brain. We found epidermal growth factor receptor (EGF-R) mRNA and protein to be expressed in infant, teen, young adult, and adult human SEZ. Some SEZ cells expressed the polysialic acid form of neural cell adhesion molecule (PSA-NCAM), characteristic of migrating neuroblasts, as well as class III β- tubulin and Hu protein, characteristic of neuroblasts and early neurons. These neuroblast-like cells were negative for glial fibrillary acidic protein (GFAP), 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNPase), and vimentin, suggesting that they were not differentiating as glia. Our results show that neuroblast-like cells exist in the human SEZ and support the theory that SEZ of postnatal human brain has neurogenic potential.",
keywords = "Class III β-tubulin, CNPase, Hu, Neu N, Neurogenesis, Precursor, PSA-NCAM, Subventricular zone",
author = "Weickert, {Cynthia Shannon} and Webster, {Maree J.} and Colvin, {Sarah M.} and Herman, {Mary M.} and Thomas Hyde and Daniel Weinberger and Joel Kleinman",
year = "2000",
month = "7",
day = "31",
doi = "10.1002/1096-9861(20000731)423:3<359::AID-CNE1>3.0.CO;2-0",
language = "English (US)",
volume = "423",
pages = "359--372",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Localization of epidermal growth factor receptors and putative neuoblasts in human subependymal zone

AU - Weickert, Cynthia Shannon

AU - Webster, Maree J.

AU - Colvin, Sarah M.

AU - Herman, Mary M.

AU - Hyde, Thomas

AU - Weinberger, Daniel

AU - Kleinman, Joel

PY - 2000/7/31

Y1 - 2000/7/31

N2 - Studies in rodents and monkeys suggest that neuronal precursor cells continue to exist and differentiate well into adulthood in these species. These results challenge the long held assumption that neurogenesis does not occur in the postnatal human brain. We examined the rostral subependymal zone (SEZ) of postnatal human brain for expression of cell phenotypic markers that have been associated with neuronal precursors and neuroblasts in rodent brain. We found epidermal growth factor receptor (EGF-R) mRNA and protein to be expressed in infant, teen, young adult, and adult human SEZ. Some SEZ cells expressed the polysialic acid form of neural cell adhesion molecule (PSA-NCAM), characteristic of migrating neuroblasts, as well as class III β- tubulin and Hu protein, characteristic of neuroblasts and early neurons. These neuroblast-like cells were negative for glial fibrillary acidic protein (GFAP), 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNPase), and vimentin, suggesting that they were not differentiating as glia. Our results show that neuroblast-like cells exist in the human SEZ and support the theory that SEZ of postnatal human brain has neurogenic potential.

AB - Studies in rodents and monkeys suggest that neuronal precursor cells continue to exist and differentiate well into adulthood in these species. These results challenge the long held assumption that neurogenesis does not occur in the postnatal human brain. We examined the rostral subependymal zone (SEZ) of postnatal human brain for expression of cell phenotypic markers that have been associated with neuronal precursors and neuroblasts in rodent brain. We found epidermal growth factor receptor (EGF-R) mRNA and protein to be expressed in infant, teen, young adult, and adult human SEZ. Some SEZ cells expressed the polysialic acid form of neural cell adhesion molecule (PSA-NCAM), characteristic of migrating neuroblasts, as well as class III β- tubulin and Hu protein, characteristic of neuroblasts and early neurons. These neuroblast-like cells were negative for glial fibrillary acidic protein (GFAP), 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNPase), and vimentin, suggesting that they were not differentiating as glia. Our results show that neuroblast-like cells exist in the human SEZ and support the theory that SEZ of postnatal human brain has neurogenic potential.

KW - Class III β-tubulin

KW - CNPase

KW - Hu

KW - Neu N

KW - Neurogenesis

KW - Precursor

KW - PSA-NCAM

KW - Subventricular zone

UR - http://www.scopus.com/inward/record.url?scp=0034738747&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034738747&partnerID=8YFLogxK

U2 - 10.1002/1096-9861(20000731)423:3<359::AID-CNE1>3.0.CO;2-0

DO - 10.1002/1096-9861(20000731)423:3<359::AID-CNE1>3.0.CO;2-0

M3 - Article

C2 - 10870078

AN - SCOPUS:0034738747

VL - 423

SP - 359

EP - 372

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 3

ER -