Localization of chemoattractant receptors on Dictyostelium discoideum cells during aggregation and down-regulation

Mei Wang, Peter J M Van Haastert, Peter N Devreotes, Pauline Schaap

Research output: Contribution to journalArticle

Abstract

cAMP chemoattractant receptors on the surface of Dictyostelium discoideum cells are visualized by means of immunocytochemistry. Receptor antigen is virtually absent from growing cells and begins to accumulate after about 6 hr of starvation, concomitant with the increase in surface cAMP binding activity. In aggregating cells, the antigen is uniformly distributed over the cell surface. Persistent cAMP stimulation, which leads to down-regulation of cAMP binding activity, induces a striking rearrangement of receptor antigen into patches or internal vesicles. A similar patching of receptor antigen is observed during tight aggregate formation, when surface cAMP binding activity decreases. These observations indicate that receptor down-regulation involves receptor agglomeration and suggest that receptor down-regulation takes place in vivo, when tight aggregates are being formed.

Original languageEnglish (US)
Pages (from-to)72-77
Number of pages6
JournalDevelopmental Biology
Volume128
Issue number1
DOIs
Publication statusPublished - 1988

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ASJC Scopus subject areas

  • Developmental Biology

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