TY - JOUR
T1 - Localization of chemoattractant receptors on Dictyostelium discoideum cells during aggregation and down-regulation
AU - Wang, Mei
AU - Van Haastert, Peter J.M.
AU - Devreotes, Peter N.
AU - Schaap, Pauline
PY - 1988/7
Y1 - 1988/7
N2 - cAMP chemoattractant receptors on the surface of Dictyostelium discoideum cells are visualized by means of immunocytochemistry. Receptor antigen is virtually absent from growing cells and begins to accumulate after about 6 hr of starvation, concomitant with the increase in surface cAMP binding activity. In aggregating cells, the antigen is uniformly distributed over the cell surface. Persistent cAMP stimulation, which leads to down-regulation of cAMP binding activity, induces a striking rearrangement of receptor antigen into patches or internal vesicles. A similar patching of receptor antigen is observed during tight aggregate formation, when surface cAMP binding activity decreases. These observations indicate that receptor down-regulation involves receptor agglomeration and suggest that receptor down-regulation takes place in vivo, when tight aggregates are being formed.
AB - cAMP chemoattractant receptors on the surface of Dictyostelium discoideum cells are visualized by means of immunocytochemistry. Receptor antigen is virtually absent from growing cells and begins to accumulate after about 6 hr of starvation, concomitant with the increase in surface cAMP binding activity. In aggregating cells, the antigen is uniformly distributed over the cell surface. Persistent cAMP stimulation, which leads to down-regulation of cAMP binding activity, induces a striking rearrangement of receptor antigen into patches or internal vesicles. A similar patching of receptor antigen is observed during tight aggregate formation, when surface cAMP binding activity decreases. These observations indicate that receptor down-regulation involves receptor agglomeration and suggest that receptor down-regulation takes place in vivo, when tight aggregates are being formed.
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U2 - 10.1016/0012-1606(88)90268-0
DO - 10.1016/0012-1606(88)90268-0
M3 - Article
C2 - 2838350
AN - SCOPUS:0023891784
SN - 0012-1606
VL - 128
SP - 72
EP - 77
JO - Developmental biology
JF - Developmental biology
IS - 1
ER -