TY - JOUR
T1 - Localization of basic fibroblast growth factor to the developing capillaries of the bovine retina
AU - Hanneken, Anne
AU - Lutty, Gerard A.
AU - McLeod, D. Scott
AU - Robey, Frank
AU - Harvey, Anita K.
AU - Hjelmeland, Leonard M.
PY - 1989/1
Y1 - 1989/1
N2 - The basic fibroblast growth factor (FGF) is a potent mitogen that has vascular endothelium as one of its principle target cells. Recent work has provided both the complete amino acid sequence of basic FGF and the nucleotide sequence of the genes for both human and bovine basic FGF. Although capillary endothelial cells have been shown to produce basic FGF in vitro and to deposit basic FGF in their extracellular matrix in vitro as well, no direct evidence yet exists for the distribution of basic FGF in vivo. Antipeptide antibodies were prepared against a 15‐amino‐acid sequence from the amino terminus of basic FGF in order to avoid cross‐reactivity with acidic FGF, a protein with 55% overall homology to basic FGF. After affinity purification, these antisera were used to localize the basic fibroblast growth factor in the fetal and adult bovine retina. Immunoreactive material was found in capillaries of the inner nuclear layer, a capillary network undergoing development during the third trimester in the fetal bovine eye. Although the resolution of the technique does not permit a unique assignment of cellular localization, the presence of stain immediately adjacent to the lumen of capillaries suggests that capillary endothelial cells may produce the basic fibroblast growth factor in vivo during vascular development.
AB - The basic fibroblast growth factor (FGF) is a potent mitogen that has vascular endothelium as one of its principle target cells. Recent work has provided both the complete amino acid sequence of basic FGF and the nucleotide sequence of the genes for both human and bovine basic FGF. Although capillary endothelial cells have been shown to produce basic FGF in vitro and to deposit basic FGF in their extracellular matrix in vitro as well, no direct evidence yet exists for the distribution of basic FGF in vivo. Antipeptide antibodies were prepared against a 15‐amino‐acid sequence from the amino terminus of basic FGF in order to avoid cross‐reactivity with acidic FGF, a protein with 55% overall homology to basic FGF. After affinity purification, these antisera were used to localize the basic fibroblast growth factor in the fetal and adult bovine retina. Immunoreactive material was found in capillaries of the inner nuclear layer, a capillary network undergoing development during the third trimester in the fetal bovine eye. Although the resolution of the technique does not permit a unique assignment of cellular localization, the presence of stain immediately adjacent to the lumen of capillaries suggests that capillary endothelial cells may produce the basic fibroblast growth factor in vivo during vascular development.
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U2 - 10.1002/jcp.1041380116
DO - 10.1002/jcp.1041380116
M3 - Article
C2 - 2910880
AN - SCOPUS:0024588955
SN - 0021-9541
VL - 138
SP - 115
EP - 120
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
IS - 1
ER -