Localization of a primate specific esterase using immunofluorescence and immunoperoxidase techniques

G. D. Vladutiu, P. E. Bigazzi, N. R. Rose

Research output: Contribution to journalArticlepeer-review

Abstract

The techniques of mixed antibody immunoperoxidase and indirect immunofluorescence were used for the localization of an esterase isoenzyme in human and monkey tissue culture cells and in monkey kidney frozen sections. In cultured cells, the esterase was found to be cytoplasmic in close apposition to the nucleus with a distribution resembling that of the Golgi region. The esterase in monkey kidney was found predominantly in the cytoplasm of the proximal convoluted tubules. There was slight staining in the Bowman's capsule of the glomerulus and in the collecting tubules of the medulla. It is suggested that the kidney is actually producing the esterase in its proximal tubule cells.

Original languageEnglish (US)
Pages (from-to)559-567
Number of pages9
JournalJournal of Histochemistry and Cytochemistry
Volume21
Issue number6
DOIs
StatePublished - 1973

ASJC Scopus subject areas

  • Anatomy
  • Histology

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