Localization of a murine oncornavirus 15,000-dalton virion protein on the membrane of neoplastic cells: Analysis by immunofluorescence and immunoelectron microscopy

O. M. Lejneva, G. I. Abelev, N. A. Dorfman, M. Strand, J. T. August

Research output: Contribution to journalArticlepeer-review

Abstract

A protein analogous to a 15,000-dalton structural protein of Rauscher murine oncornavirus has been localized on cell membranes of Rauscher erythroblastosis and CC57BR murine lymphosarcoma cells by use of monospecific antiserum in conjunction with immunofluorescence and immunoelectron microscopy techniques. Antigenic determinants of this protein were present on the membranes of erythroblastosis and lymphosarcoma cells, but were absent from the membrane of budding or mature virions. Both typespecific and group-specific determinants of the protein were accessible to antibodies reacting at the cell surface. This viral protein, expressed on the cell membrane, appears to correspond to previously described cell surface antigens of virus-induced murine leukemias.

Original languageEnglish (US)
Pages (from-to)281-292
Number of pages12
JournalVirology
Volume75
Issue number2
DOIs
StatePublished - Dec 1976

ASJC Scopus subject areas

  • Virology

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