Localization of a human gene homologous to the PrP gene on the p ARM of chromosome 20 and detection of PrP-related antigens in normal human brain

Nikolaos K. Robakis, Evelyn A. Devine-Gage, Edmund C. Jenkins, Richard J. Kascsak, W. Ted Brown, Michael S. Krawczun, Wayne P. Silverman

Research output: Contribution to journalArticlepeer-review

Abstract

Infectious fractions prepared from scrapie-infected hamster brains contain a protein, PrP 27-30, which shares antigenic determinants with polypeptides found in similarly prepared fractions from patients with Creutzfeldt-Jakob disease. cDNA sequences encoding the hamster PrP 27-30 identified homologous sequences in the human genome as well as in normal human brain mRNA preparations. Antibodies raised against the mouse PrP's identified antigenically related peptides in both normal hamster and human brain as well as in scrapie-infected hamster brain and CJD-affected human brain. By using in situ hybridization we localized the homologous human genomic sequences on the short arm of chromosome 20. Our results indicate that the reportedly unique proteins detected in human CJD preparations derive from normal human gene products.

Original languageEnglish (US)
Pages (from-to)758-765
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume140
Issue number2
DOIs
StatePublished - Oct 30 1986

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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