Localization and imaging of sialylated glycosphingolipids in brain tissue sections by MALDI mass spectrometry

Benoit Colsch, Amina S. Woods

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we describe a simple and efficient method for mapping the distribution and localization of all sialylated sphingoglycolipids present in coronal mouse brain sections using a conventional axial matrix-assisted laser desorption/ionization time of flight. A single scan of a histological tissue section gives a complete profile of ganglioside species without derivatization or labeling. We have developed and tested a new matrix preparation (2,6-dihydroxyacetophenone [DHA]/ammonium sulfate/heptafluorobutyric acid [HFBA]) to maximize the detection of all ganglioside species; the ammonium sulfate limits the formation of salt adducts, while the addition of HFBA increases the stability of DHA in a vacuum, thus facilitating imaging applications. Our results, in both extracted samples and whole tissue sections using negative ion reflectron and linear modes, show differences in localization in several brain regions depending on the sialic acids and the ceramide-associated core gangliosides.

Original languageEnglish (US)
Article numbercwq031
Pages (from-to)661-667
Number of pages7
JournalGlycobiology
Volume20
Issue number6
DOIs
StatePublished - Feb 28 2010
Externally publishedYes

Keywords

  • Brain
  • Gangliosides
  • Imaging
  • MALDI
  • Mass spectrometry

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Localization and imaging of sialylated glycosphingolipids in brain tissue sections by MALDI mass spectrometry'. Together they form a unique fingerprint.

Cite this