Local release of B cell-activating factor of the TNF family after segmental allergen challenge of allergic subjects

Atsushi Kato, Huiqing Xiao, Regina T. Chustz, Mark Chang Hwa Liu, Robert P. Schleimer

Research output: Contribution to journalArticle

Abstract

Background: Local production of IgA and IgE in the airways has been proposed to be an important event in both immune protection from pathogens and the pathogenesis of airway allergic diseases. Objective: The objective of this study was to investigate the production of B cell-activating factor of the TNF family (BAFF), an important regulator of B-cell survival and immunoglobulin class-switch recombination, in bronchoalveolar lavage (BAL) fluid after segmental allergen challenge of allergic subjects. Methods: Segmental allergen challenge with saline or allergen was performed in 16 adult allergic subjects. BAL was performed at both saline- and allergen-challenged sites 20 to 24 hours after challenge. Concentrations of B cell-active cytokines, including BAFF, IL-6, and IL-13, were measured by using specific ELISA and cytometric bead array assays. Results: Levels of BAFF protein were significantly increased in BAL fluid after allergen challenge (53.8 pg/mL [range, 0-407.4 pg/mL], P = .001) compared with those at saline-challenged sites (0 pg/mL [0-34.7 pg/mL]). In the BAL fluid after allergen challenge, BAFF levels were significantly correlated with absolute numbers of total cells (r = 0.779, P <.001), lymphocytes (r = 0.842, P <.001), neutrophils (r = 0.809, P <.001), and eosinophils (r = 0.621, P = .010) but did not correlate with macrophages. Normalization to albumin indicated that BAFF production occurred locally in the airways. BAFF levels were also significantly correlated with the other B cell-activating cytokines IL-6 (r = 0.875, P <.001) and IL-13 (r = 0.812, P <.001). Conclusion: The antigen-induced production of BAFF in the airway might contribute to local class-switch recombination and immunoglobulin synthesis by B cells.

Original languageEnglish (US)
JournalThe Journal of Allergy and Clinical Immunology
Volume123
Issue number2
DOIs
StatePublished - Feb 2009

Fingerprint

B-Cell Activating Factor
Allergens
Bronchoalveolar Lavage Fluid
B-Lymphocytes
Interleukin-13
Genetic Recombination
Interleukin-6
Cytokines
Immunoglobulin Isotypes
Bronchoalveolar Lavage
Eosinophils
Immunoglobulin A
Immunoglobulin E
Albumins
Cell Survival
Neutrophils
Cell Count
Enzyme-Linked Immunosorbent Assay
Macrophages
Lymphocytes

Keywords

  • allergic diseases
  • B cell-activating factor of the TNF family
  • B cells
  • eosinophils
  • Local immunoglobulin production
  • segmental allergen challenge

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Local release of B cell-activating factor of the TNF family after segmental allergen challenge of allergic subjects. / Kato, Atsushi; Xiao, Huiqing; Chustz, Regina T.; Liu, Mark Chang Hwa; Schleimer, Robert P.

In: The Journal of Allergy and Clinical Immunology, Vol. 123, No. 2, 02.2009.

Research output: Contribution to journalArticle

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keywords = "allergic diseases, B cell-activating factor of the TNF family, B cells, eosinophils, Local immunoglobulin production, segmental allergen challenge",
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AU - Xiao, Huiqing

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AU - Liu, Mark Chang Hwa

AU - Schleimer, Robert P.

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AB - Background: Local production of IgA and IgE in the airways has been proposed to be an important event in both immune protection from pathogens and the pathogenesis of airway allergic diseases. Objective: The objective of this study was to investigate the production of B cell-activating factor of the TNF family (BAFF), an important regulator of B-cell survival and immunoglobulin class-switch recombination, in bronchoalveolar lavage (BAL) fluid after segmental allergen challenge of allergic subjects. Methods: Segmental allergen challenge with saline or allergen was performed in 16 adult allergic subjects. BAL was performed at both saline- and allergen-challenged sites 20 to 24 hours after challenge. Concentrations of B cell-active cytokines, including BAFF, IL-6, and IL-13, were measured by using specific ELISA and cytometric bead array assays. Results: Levels of BAFF protein were significantly increased in BAL fluid after allergen challenge (53.8 pg/mL [range, 0-407.4 pg/mL], P = .001) compared with those at saline-challenged sites (0 pg/mL [0-34.7 pg/mL]). In the BAL fluid after allergen challenge, BAFF levels were significantly correlated with absolute numbers of total cells (r = 0.779, P <.001), lymphocytes (r = 0.842, P <.001), neutrophils (r = 0.809, P <.001), and eosinophils (r = 0.621, P = .010) but did not correlate with macrophages. Normalization to albumin indicated that BAFF production occurred locally in the airways. BAFF levels were also significantly correlated with the other B cell-activating cytokines IL-6 (r = 0.875, P <.001) and IL-13 (r = 0.812, P <.001). Conclusion: The antigen-induced production of BAFF in the airway might contribute to local class-switch recombination and immunoglobulin synthesis by B cells.

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