Local generation of sulfidopeptide leukotrienes upon nasal provocation with cold, dry air

A. G. Togias, R. M. Naclerio, S. P. Peters, I. Nimmagadda, D. Proud, A. Kagey-Sobotka, N Franklin Adkinson, Philip S. Norman, L. M. Lichtenstein

Research output: Contribution to journalArticle

Abstract

In order to assess whether sulfidopeptide leukotrienes are generated following nonimmunologic stimulation of inflammatory cells in vivo, 11 subjects complaining of symptoms of rhinitis when exposed to cold and dry environments were challenged by nasal breathing, first with warm, moist air and then with cold, dry air. Nasal lavages with normal saline were performed before and after each exposure. Immunoreactive leukotriene in the lavage fluids was significantly increased following cold, dry air exposure (2.6 ng/ml) compared with that after warm, moist air exposure (0.7 ng/ml) or at baseline (0.4 ng/ml) (p <0.01 in both instances). Six more subjects, denying cold-air sensitivity, were subjected to the same protocol and had no mediator and symptom score changes after cold, dry air challenge. Leukotriene changes after cold, dry air were highly concordant with increments in histamine, prostaglandin D2,N-α-tosyl-L-arginine methyl ester (TAME) esterase(s) activity and symptom scores (p <0.001). Separation of leukotrienes by high performance liquid chromatography in the nasal washes of 3 subjects showed variable amounts of LTC4, D4, and E4, suggesting metabolism of the former to the latter 2. To our knowledge, this is the first in vivo demonstration of leukotriene production in response to a physical stimulus, and it suggests a possible role of these and other inflammatory mediators in pathologic conditions, such as exercise-induced asthma, that involve physical causative factors.

Original languageEnglish (US)
Pages (from-to)1133-1137
Number of pages5
JournalAmerican Review of Respiratory Disease
Volume133
Issue number6
StatePublished - 1986
Externally publishedYes

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Leukotrienes
Nose
Air
Nasal Lavage
Exercise-Induced Asthma
Prostaglandin D2
Leukotriene C4
Therapeutic Irrigation
Rhinitis
Histamine
Respiration
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Togias, A. G., Naclerio, R. M., Peters, S. P., Nimmagadda, I., Proud, D., Kagey-Sobotka, A., ... Lichtenstein, L. M. (1986). Local generation of sulfidopeptide leukotrienes upon nasal provocation with cold, dry air. American Review of Respiratory Disease, 133(6), 1133-1137.

Local generation of sulfidopeptide leukotrienes upon nasal provocation with cold, dry air. / Togias, A. G.; Naclerio, R. M.; Peters, S. P.; Nimmagadda, I.; Proud, D.; Kagey-Sobotka, A.; Adkinson, N Franklin; Norman, Philip S.; Lichtenstein, L. M.

In: American Review of Respiratory Disease, Vol. 133, No. 6, 1986, p. 1133-1137.

Research output: Contribution to journalArticle

Togias, AG, Naclerio, RM, Peters, SP, Nimmagadda, I, Proud, D, Kagey-Sobotka, A, Adkinson, NF, Norman, PS & Lichtenstein, LM 1986, 'Local generation of sulfidopeptide leukotrienes upon nasal provocation with cold, dry air', American Review of Respiratory Disease, vol. 133, no. 6, pp. 1133-1137.
Togias AG, Naclerio RM, Peters SP, Nimmagadda I, Proud D, Kagey-Sobotka A et al. Local generation of sulfidopeptide leukotrienes upon nasal provocation with cold, dry air. American Review of Respiratory Disease. 1986;133(6):1133-1137.
Togias, A. G. ; Naclerio, R. M. ; Peters, S. P. ; Nimmagadda, I. ; Proud, D. ; Kagey-Sobotka, A. ; Adkinson, N Franklin ; Norman, Philip S. ; Lichtenstein, L. M. / Local generation of sulfidopeptide leukotrienes upon nasal provocation with cold, dry air. In: American Review of Respiratory Disease. 1986 ; Vol. 133, No. 6. pp. 1133-1137.
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