Local anesthetics inhibit endothelium-dependent vasodilation

Research output: Contribution to journalArticle

Abstract

The effect of local anesthetics on endothelium-dependent and endothelium-independent vasodilation was determined using isolated rat thoracic aorta. Endothelium-intact rat aortic rings were mounted for isometric tension recording. Cumulative concentration-dependent vascular relaxation responses to the endothelium-dependent vasodilators methacholine (1 x 10-8 to 3 x 10-5 M), the calcium ionophore A23187 (1 x 10-8 to 3 x 10-7 M) and the endothelium-independent vasodilator sodium nitroprusside (1 x 10-9 to 1 x 10-7 M) were determined in the presence or absence of bupivacaine, lidocaine, etidocaine, or 2-chloroprocaine (1 x 10-4 M). All of the local anesthetics studied significantly (P <0.05) inhibited endothelium-dependent relaxations to the receptor-mediated methacholine and the nonreceptor-mediated A23187. Bupivacaine was more potent (P <0.01) than the other three anesthetics tested. Direct, endothelium-independent vasodilation by sodium nitroprusside was not affected. The local anesthetics appear to exert their inhibitory effect on endothelium-dependent vasodilation at a site distal to receptor activation at the endothelial cell and proximal to guanylate cyclase activation in the vascular smooth muscle.

Original languageEnglish (US)
Pages (from-to)805-811
Number of pages7
JournalAnesthesiology
Volume70
Issue number5
StatePublished - 1989
Externally publishedYes

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Local Anesthetics
Vasodilation
Endothelium
Methacholine Chloride
Bupivacaine
Calcimycin
Nitroprusside
Etidocaine
Endothelium-Dependent Relaxing Factors
Calcium Ionophores
Guanylate Cyclase
Lidocaine
Thoracic Aorta
Vasodilator Agents
Vascular Smooth Muscle
Blood Vessels
Anesthetics
Endothelial Cells

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Local anesthetics inhibit endothelium-dependent vasodilation. / Johns, Roger A.

In: Anesthesiology, Vol. 70, No. 5, 1989, p. 805-811.

Research output: Contribution to journalArticle

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