Local administration of granulocyte/macrophage colony-stimulating factor increases the number and activation state of dendritic cells in the sentinel lymph node of early-stage melanoma

Ronald J C L M Vuylsteke, Barbara G. Molenkamp, Hester A. Gietema, Paul A M Van Leeuwen, Pepijn G J T B Wijnands, Wim Vos, Paul J. Van Diest, Rik J. Scheper, Sybren Meijer, Tanja D. De Gruijl

Research output: Contribution to journalArticlepeer-review

Abstract

The initial tumor-draining lymph node, the sentinel lymph node, not only constitutes the first expected site of micrometastasis but also the first point of contact between tumor-associated antigens and the adaptive immune system. A tumor-induced decrease in the frequency and activation state of sentinel lymph node dendritic cells will impair the generation of effective antitumor T-cell responses and increase the likelihood of metastatic spread. Here, we demonstrate that intradermal administration of granulocyte macrophage-colony stimulating factor around the excision site of stage I primary melanoma tumors increases the number and activation state of dendritic cells in the paracortical areas of the sentinel lymph node and enhances their binding to T cells. We conclude that local treatment of melanoma patients with granulocyte macrophage-colony stimulating factor, before surgery, conditions the sentinel lymph node microenvironment to enhance mature dendritic cell recruitment and hypothesize that this may be more conducive to the generation of T-cell-mediated antitumor immunity.

Original languageEnglish (US)
Pages (from-to)8456-8460
Number of pages5
JournalCancer Research
Volume64
Issue number22
DOIs
StatePublished - Nov 15 2004
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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