TY - JOUR
T1 - Local administration of granulocyte/macrophage colony-stimulating factor increases the number and activation state of dendritic cells in the sentinel lymph node of early-stage melanoma
AU - Vuylsteke, Ronald J C L M
AU - Molenkamp, Barbara G.
AU - Gietema, Hester A.
AU - Van Leeuwen, Paul A M
AU - Wijnands, Pepijn G J T B
AU - Vos, Wim
AU - Van Diest, Paul J.
AU - Scheper, Rik J.
AU - Meijer, Sybren
AU - De Gruijl, Tanja D.
PY - 2004/11/15
Y1 - 2004/11/15
N2 - The initial tumor-draining lymph node, the sentinel lymph node, not only constitutes the first expected site of micrometastasis but also the first point of contact between tumor-associated antigens and the adaptive immune system. A tumor-induced decrease in the frequency and activation state of sentinel lymph node dendritic cells will impair the generation of effective antitumor T-cell responses and increase the likelihood of metastatic spread. Here, we demonstrate that intradermal administration of granulocyte macrophage-colony stimulating factor around the excision site of stage I primary melanoma tumors increases the number and activation state of dendritic cells in the paracortical areas of the sentinel lymph node and enhances their binding to T cells. We conclude that local treatment of melanoma patients with granulocyte macrophage-colony stimulating factor, before surgery, conditions the sentinel lymph node microenvironment to enhance mature dendritic cell recruitment and hypothesize that this may be more conducive to the generation of T-cell-mediated antitumor immunity.
AB - The initial tumor-draining lymph node, the sentinel lymph node, not only constitutes the first expected site of micrometastasis but also the first point of contact between tumor-associated antigens and the adaptive immune system. A tumor-induced decrease in the frequency and activation state of sentinel lymph node dendritic cells will impair the generation of effective antitumor T-cell responses and increase the likelihood of metastatic spread. Here, we demonstrate that intradermal administration of granulocyte macrophage-colony stimulating factor around the excision site of stage I primary melanoma tumors increases the number and activation state of dendritic cells in the paracortical areas of the sentinel lymph node and enhances their binding to T cells. We conclude that local treatment of melanoma patients with granulocyte macrophage-colony stimulating factor, before surgery, conditions the sentinel lymph node microenvironment to enhance mature dendritic cell recruitment and hypothesize that this may be more conducive to the generation of T-cell-mediated antitumor immunity.
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U2 - 10.1158/0008-5472.CAN-03-3251
DO - 10.1158/0008-5472.CAN-03-3251
M3 - Article
C2 - 15548718
AN - SCOPUS:8544239395
SN - 0008-5472
VL - 64
SP - 8456
EP - 8460
JO - Cancer Research
JF - Cancer Research
IS - 22
ER -